PMID: 32521908Jun 12, 2020Paper

Nobiletin flavone inhibits the growth and metastasis of human pancreatic cancer cells via induction of autophagy, G0/G1 cell cycle arrest and inhibition of NF-kB signalling pathway

Journal of B.U.ON. : Official Journal of the Balkan Union of Oncology
Hao JiangHaiou Wang

Abstract

The anticancer effects of nobiletin have not been fully explored against the human pancreatic cancer cells. Therefore this study was undertaken to evaluate the anticancer effects of nobiletin against the MIAPaCa-2 human pancreatic cancer cells along with evaluating its effects on autophagy, cell cycle phase distribution, cell migration and invasion and NF-kB signalling pathway. Cell proliferation was evaluated by CCK-8 assay while cell cycle analysis was carried out by flow cytometry. Effects on cell migration and invasion were evaluated by wound healing assay and transwell assays respectively. Transmission electron microscopy (TEM) and western blot were used to study the effects on autophagy and NF-kB signalling pathway. The results revealed that nobiletin restrained the proliferation rate of the MIAPaCa-2 human pancreatic cancer cells and showed an IC50 of 6.12 µM. However, nobiletin exhibited very high IC50 against the normal ms-1 pancreatic cells. TEM showed that nobiletin triggered autophagy in the MIAPaCa-2 cancer cells which was accompanied by enhancement in the expression of LC3B II and LC3-I, and decrease in the expression of p62. Cell cycle analysis showed that nobiletin caused accretion of the MIAPaCa-2 cells in the ...Continue Reading

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