Nocardia isolation from clinical samples with the paraffin baiting technique

Germs
Mehdi Fatahi BafghiSeyyed Saeed Eshraghi

Abstract

The genus Nocardia is a cause of infection in the lungs, skin, brain, cerebrospinal fluid, eyes, joints and kidneys. Nocardia isolation from polymicrobial specimens is difficult due to its slow growth. Several methods have been reported for Nocardia isolation from clinical samples. In the current study, we used three methods: paraffin baiting technique, paraffin agar, and conventional media for Nocardia isolation from various clinical specimens from Iranian patients. In this study, we examined 517 samples from various clinical specimens such as: sputum of patients with suspected tuberculosis, bronchoalveolar lavage, sputum of patients with cystic fibrosis, tracheal aspirate, cutaneous and subcutaneous abscesses, cerebrospinal fluid, dental abscess, mycetoma, wound, bone marrow biopsy, and gastric lavage. All collected specimens were cultured on carbon-free broth tubes (paraffin baiting technique), paraffin agar, Sabouraud dextrose agar, and Sabouraud dextrose agar with cycloheximide and were incubated at 35°C for one month. Seven Nocardia spp. were isolated with paraffin baiting technique, compared with 5 positive results with the paraffin agar technique and 3 positive results with Sabouraud dextrose agar with and without cyclo...Continue Reading

Citations

Jan 13, 2017·Circulation Journal : Official Journal of the Japanese Circulation Society·Mehdi Fatahi-Bafghi
Oct 12, 2016·Microbiology Spectrum·A Brian MochonMichael A Saubolle
Jun 1, 2021·Japanese Journal of Infectious Diseases·Jun LiMing-Xiang Zou

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