Nocardia polymerase chain reaction (PCR)-based assay performed on bronchoalveolar lavage fluid after lung transplantation: A prospective pilot study

PloS One
Julien CoussementVerónica Rodríguez-Nava

Abstract

Transplant recipients are at risk of pulmonary nocardiosis, a life-threatening opportunistic infection caused by Nocardia species. Given the limitations of conventional diagnostic techniques (i.e., microscopy and culture), a polymerase chain reaction (PCR)-based assay was developed to detect Nocardia spp. on clinical samples. While this test is increasingly being used by transplant physicians, its performance characteristics are not well documented. We evaluated the performance characteristics of this test on bronchoalveolar lavage (BAL) fluid samples from lung transplant recipients (LTRs). We prospectively included all BAL samples from LTRs undergoing bronchoscopy at our institution between December 2016 and June 2017 (either surveillance or clinically-indicated bronchoscopies). Presence of microbial pathogens was assessed using techniques available locally (including microscopy and 10-day culture for Nocardia). BAL samples were also sent to the French Nocardiosis Observatory (Lyon, France) for the Nocardia PCR-based assay. Transplant physicians and patients were blinded to the Nocardia PCR results. We included 29 BAL samples from 21 patients (18 surveillance and 11 clinically-indicated bronchoscopies). Nocardiosis was not dia...Continue Reading

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Citations

Jul 4, 2020·Current Opinion in Organ Transplantation·Jennifer L Saullo, Rachel A Miller
Dec 22, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ili MargalitElad Goldberg
Oct 28, 2020·Expert Review of Respiratory Medicine·Lorena van den BogaartOriol Manuel
Jan 9, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Ili MargalitJulien Coussement
Dec 31, 2020·Infection and Drug Resistance·Emmanuel LafontDavid Lebeaux
Aug 22, 2021·Journal of Clinical Laboratory Analysis·Yumeng PengYumei Ge
Sep 21, 2021·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Moritz Z KayserJens Gottlieb
Sep 9, 2021·Current Opinion in Infectious Diseases·David LebeauxPierre Tattevin

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Methods Mentioned

BETA
PCRs
PCR
bronchoalveolar lavage
biopsy

Software Mentioned

blast

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