Nociceptin/orphanin FQ suppresses adaptive immune responses in vivo and at picomolar levels in vitro.

Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology
Benito AntonToby K Eisenstein

Abstract

Nociceptin/orphanin FQ (N/OFQ), added in vitro to murine spleen cells in the picomolar range, suppressed antibody formation to sheep red blood cells in a primary and a secondary plaque-forming cell assay. The activity of the peptide was maximal at 10(-12) M, with an asymmetric U-shaped dose-response curve that extended activity to 10(-14) M. Suppression was not blocked by pretreatment with naloxone. Specificity of the suppressive response was shown using affinity-purified rabbit antibodies against two N/OFQ peptides and with a pharmacological antagonist. Antisera against both peptides were active, in a dose-related manner, in neutralizing N/OFQ-mediated immunosuppression, when the peptide was used at concentrations from 10(-12.3) to 10(-11.6) M. In addition, nociceptin given in vivo by osmotic pump for 48 h suppressed the capacity of spleen cells placed ex vivo to make an anti-sheep red blood cell response. These studies show that nociceptin directly inhibits an adaptive immune response, i.e., antibody formation, both in vitro and in vivo.

References

Sep 7, 1979·Science·E HazumP Cuatrecasas
Jan 1, 1988·Cold Spring Harbor Symposia on Quantitative Biology·J M HorowitzE Harlow
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H M JohnsonJ E Blalock
Jun 1, 1995·Journal of Neuroimmunology·W P HalfordD J Carr
Feb 27, 1996·Biochemical and Biophysical Research Communications·T HoutaniT Sugimoto
Apr 29, 1996·The Journal of Comparative Neurology·B AntonC J Evans
Dec 13, 1996·The Journal of Biological Chemistry·F MengH Akil
Jan 27, 1998·The Journal of Biological Chemistry·R K ReinscheidO Civelli
Jun 4, 1998·Journal of Neuroimmunology·T K Eisenstein, M E Hilburger
Jul 9, 1998·Journal of Neuroimmunology·J P LaiW Z Ho
Oct 19, 1999·Synapse·J S HomG W Pasternak
Sep 22, 2000·Peptides·C PolidoriM Massi
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C N SerhanM Pouliot
Sep 13, 2002·Journal of Neuroimmunology·Jamshid ArjomandChris J Evans
Dec 27, 2003·World Journal of Gastroenterology : WJG·Ferenc SzalayKornelia Tekes
Dec 27, 2003·World Journal of Gastroenterology : WJG·Andrea HorvathFerenc Szalay
Mar 17, 2004·Journal of Neuroimmunology·Paula S WaitsJulie D McLeod
Jun 1, 2004·Pharmacological Reviews·Mario DelgadoDoina Ganea
Mar 8, 2005·Journal of Neuroimmunology·Shingo KatoSoichiro Miura

❮ Previous
Next ❯

Citations

May 7, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Courtney L DonicaKelly M Standifer
Jun 10, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·David E Kaminsky, Thomas J Rogers
Jun 26, 2013·British Journal of Anaesthesia·M Al-HashimiD G Lambert
Feb 9, 2012·Journal of Amino Acids·Elaine C Gavioli, Pedro R T Romão
Jan 29, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Carla PetrellaSimona Agostini
Oct 11, 2011·Peptides·Richard J Bodnar
Jul 17, 2013·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·A SiepertB Sawitzki
Mar 19, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nikol SulloBruno D'Agostino
May 10, 2017·Neurochemical Research·Xue Tao QiAlbert Cheung Hoi Yu
Apr 6, 2021·Scandinavian Journal of Immunology·Ann L BenkoNancy J Olsen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.