Nociceptive stimulation induces expression of Arc/Arg3.1 in the spinal cord with a preference for neurons containing enkephalin.

Molecular Pain
Mehdi HossainiJan C Holstege

Abstract

In pain processing, long term synaptic changes play an important role, especially during chronic pain. The immediate early gene Arc/Arg3.1 has been widely implicated in mediating long-term plasticity in telencephalic regions, such as the hippocampus and cortex. Accordingly, Arc/Arg3.1 knockout (KO) mice show a deficit in long-term memory consolidation. Here, we identify expression of Arc/Arg3.1 in the rat spinal cord using immunohistochemistry and in situ hybridization following pain stimuli. We found that Arc/Arg3.1 is not present in naïve or vehicle treated animals, and is de novo expressed in dorsal horn neurons after nociceptive stimulation. Expression of Arc/Arg3.1 was induced in an intensity dependent manner in neurons that were located in laminae I (14%) and II (85%) of the spinal dorsal horn. Intrathecal injection of brain derived neurotrophic factor (BDNF) also induced expression of Arc/Arg3.1. Furthermore, 90% of Arc/Arg3.1 expressing neurons also contained the activity marker c-Fos, which was expressed more abundantly. Preproenkephalin mRNA was found in the majority (68%) of the Arc/Arg3.1 expressing neurons, while NK-1 was found in only 19% and GAD67 mRNA in 3.6%. Finally, pain behavior in Arc/Arg3.1 KO mice was not...Continue Reading

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Citations

May 22, 2013·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Julia Y LjubimovaKeith L Black
Oct 11, 2011·Peptides·Richard J Bodnar
Nov 21, 2018·Life Science Alliance·Anaïs SoulaAlexandre Favereaux
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Mar 25, 2015·Molecular Psychiatry·L A Gouty-ColomerS A Kushner
Apr 24, 2021·The Journal of Pain : Official Journal of the American Pain Society·Matthew R SapioAndrew J Mannes
May 11, 2021·The Journal of Experimental Medicine·Juliana M Navia-PelaezYury I Miller
Aug 7, 2021·British Journal of Pharmacology·June Bryan de la PeñaZachary T Campbell

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