NOD/SCID-GAMMA mice are an ideal strain to assess the efficacy of therapeutic agents used in the treatment of myeloma bone disease

PloS One
M A LawsonA D Chantry

Abstract

Animal models of multiple myeloma vary in terms of consistency of onset, degree of tumour burden and degree of myeloma bone disease. Here we describe five pre-clinical models of myeloma in NOD/SCID-GAMMA mice to specifically study the effects of therapeutic agents on myeloma bone disease. Groups of 7-8 week old female irradiated NOD/SCID-GAMMA mice were injected intravenously via the tail vein with either 1x106 JJN3, U266, XG-1 or OPM-2 human myeloma cell lines or patient-derived myeloma cells. At the first signs of morbidity in each tumour group all animals were sacrificed. Tumour load was measured by histological analysis, and bone disease was assessed by micro-CT and standard histomorphometric methods. Mice injected with JJN3, U266 or OPM-2 cells showed high tumour bone marrow infiltration of the long bones with low variability, resulting in osteolytic lesions. In contrast, mice injected with XG-1 or patient-derived myeloma cells showed lower tumour bone marrow infiltration and less bone disease with high variability. Injection of JJN3 cells into NOD/SCID-GAMMA mice resulted in an aggressive, short-term model of myeloma with mice exhibiting signs of morbidity 3 weeks later. Treating these mice with zoledronic acid at the tim...Continue Reading

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Citations

Feb 26, 2016·BoneKEy Reports·Seint T LwinRebecca Silbermann
Mar 25, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michelle A LawsonR Graham G Russell
Oct 16, 2018·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Julia Paton-HoughMichelle A Lawson
Mar 29, 2019·Science Translational Medicine·Eric L SmithRenier J Brentjens
May 10, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Laura M Calvi, Lorenz C Hofbauer
May 8, 2018·Oncotarget·Marco RossiPierfrancesco Tassone
Sep 9, 2016·Immunopharmacology and Immunotoxicology·Bartholomew Okechukwu IbehLucy Ogbadu
Feb 23, 2020·JBMR Plus·Marta Diaz-delCastilloAnne-Marie Heegaard
Dec 25, 2019·Journal of Bone Oncology·Nicole A KohartThomas J Rosol
Mar 21, 2021·Journal for Immunotherapy of Cancer·Louise M E MüllerFiona Errington-Mais
May 1, 2021·Cancers·Alejandra Ortiz-RuizMiguel Gallardo
Jun 25, 2021·Frontiers in Genetics·Syed Hassan MehdiDonghoon Yoon

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Methods Mentioned

BETA
xenograft
fluorescence-activated cell sorting
FACS

Software Mentioned

ImageJ
GraphPad Instat
Drishti

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