Nodule excitability in an animal model of periventricular nodular heterotopia: c-fos activation in organotypic hippocampal slices

Epilepsia
Emily T DoisyPhilip A Schwartzkroin

Abstract

Aberrations in brain development may lead to dysplastic structures such as periventricular nodules. Although these abnormal collections of neurons are often associated with difficult-to-control seizure activity, there is little consensus regarding the epileptogenicity of the nodules themselves. Because one common treatment option is surgical resection of suspected epileptic nodules, it is important to determine whether these structures in fact give rise, or essentially contribute, to epileptic activities. To study the excitability of aberrant nodules, we have examined c-fos activation in organotypic hippocampal slice cultures generated from an animal model of periventricular nodular heterotopia created by treating pregnant rats with methylazoxymethanol acetate. Using this preparation, we have also attempted to assess tissue excitability when the nodule is surgically removed from the culture. We then compared c-fos activation in this in vitro preparation to c-fos activation generated in an intact rat treated with kainic acid. Quantitative analysis of c-fos activation failed to show enhanced nodule excitability compared to neocortex or CA1 hippocampus. However, when we compared cultures with and without a nodule, presence of a no...Continue Reading

References

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Nov 1, 1989·Trends in Neurosciences·J I Morgan, T Curran
Apr 1, 1981·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S M HsuH Fanger
May 14, 1998·Epilepsia·G BattagliaG Avanzini
Dec 7, 2000·Mental Retardation and Developmental Disabilities Research Reviews·P A Schwartzkroin, C A Walsh
Jan 22, 2005·Brain : a Journal of Neurology·Yahya AghakhaniFrançois Dubeau
Dec 1, 2006·Seizure : the Journal of the British Epilepsy Association·H StefanJ Romstöck
Dec 7, 2006·Developmental Neuroscience·H Jurgen WenzelPhilip A Schwartzkroin
Nov 23, 2007·Clinical Neurophysiology : Official Journal of the International Federation of Clinical Neurophysiology·Luc ValtonFabrice Bartolomei
Feb 2, 2011·Brain Research·Anna KarlssonFredrik Asztely
Sep 22, 2011·Epilepsia·Naranzogt TschuluunPhilip A Schwartzkroin
Sep 24, 2013·Brain : a Journal of Neurology·Valerio ContiRenzo Guerrini
Oct 5, 2013·Epilepsy & Behavior : E&B·Joanna A ChristodoulouBernard S Chang
May 28, 2014·Epilepsia·Isaac Marin-ValenciaJoseph G Gleeson
Jul 31, 2014·Annals of Neurology·Ludovic Franck PetitJean-Bernard Manent

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Citations

Apr 8, 2015·Journal of Neuroscience Methods·Heiko J Luhmann
Aug 22, 2016·Seminars in Pediatric Neurology·Tarek ShakerLionel Carmant

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