Noggin underexpression and runx-2 overexpression in a craniosynostosis rabbit model

Annals of Plastic Surgery
Joubin S GabbayJames P Bradley

Abstract

Normal suture fusion has been shown to be driven by the molecular signals elucidated by the underlying dura. However, the pathogenesis of suture fusion in craniosynostosis is not well described. The purpose of our study was to examine the expression patterns of 2 important molecular signals (Noggin and Runx-2) in a cohort of congenital craniosynostotic rabbits to gain a better understanding of suture behavior in craniosynostosis. Coronal (fusing) and sagittal (patent) rabbit cranial sutures from a colony of congenitally synostosed rabbits and wild-type (control) rabbits were harvested at a neonatal time point. These sections were then grown in organ culture and harvested for histology at 0, 7, or 14 days of culture. Fusion percentage was then assessed and an overall fusion score was calculated. Expression of Noggin and Runx-2 was then localized by immunohistochemistry and quantified by Western blot analysis. Histology of the wild-type cranial sutures (control) showed suture patency (score of 0%) for all coronal and sagittal sutures at 0 days, 7 days, and 14 days of organ culture. Sagittal sutures of craniosynostotic animals also showed suture patency (score of 0%) at all culture times (0, 7, and 14 days). Of the 18 coronal sutu...Continue Reading

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Citations

Oct 24, 2008·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Georges Louis Kaiser, Valérie Oesch
Nov 21, 2007·Bone·Eduardo K MoioliJeremy J Mao
Apr 18, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gregory M CooperMark P Mooney
May 15, 2009·Clinical Implant Dentistry and Related Research·Omar OmarPeter Thomsen
Apr 2, 2016·Journal of Neurosurgery. Pediatrics·Alaina WhittonRene Olivares-Navarrete

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