Nogo-receptor 1 deficiency has no influence on immune cell repertoire or function during experimental autoimmune encephalomyelitis

PloS One
Sara A LitwakClaude C A Bernard

Abstract

The potential role of Nogo-66 Receptor 1 (NgR1) on immune cell phenotypes and their activation during neuroinflammatory diseases such as multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE), is unclear. To further understand the function of this receptor on haematopoietically-derived cells, phenotypic and functional analyses were performed using NgR1-deficient (ngr1-/-) animals. Flow cytometry-based phenotypic analyses performed on blood, spleen, thymus, lymph nodes, bone marrow and central nervous-system (CNS)-infiltrating blood cells revealed no immunological defects in naïve ngr1-/- animals versus wild-type littermate (WTLM) controls. EAE was induced by either recombinant myelin oligodendrocyte glycoprotein (rMOG), a model in which B cells are considered to contribute pathogenically, or by MOG35-55 peptide, a B cell-independent model. We have demonstrated that in ngr1-/- mice injected with MOG35-55, a significant reduction in the severity of EAE correlated with reduced axonal damage present in the spinal cord when compared to their WTLM controls. However, despite a reduction in axonal damage observed in the CNS of ngr1-/- mice at the chronic stage of disease, no clinical differences c...Continue Reading

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Citations

Sep 9, 2015·Biochemical Pharmacology·Stefanie SeilerHans Rudolf Widmer
Apr 7, 2016·Journal of Neuroimmunology·Sandra KuehnStephanie C Joachim
Jan 3, 2018·Molecular Neurobiology·Paschalis Theotokis, Nikolaos Grigoriadis
May 20, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eduardo HuarteDavid W Pascual

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Methods Mentioned

BETA
PCR
fluorescence-activated cell sorting
FACS
fluorescence imaging
ELISA
flow cytometry

Software Mentioned

Prism
ImageJ
Soft Flow
GraphPad
FACSDiva
FCAP
Adobe Photoshop

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