Non-apoptotic desquamation of cells from corneal epithelium: putative role for Muc4/sialomucin complex in cell release and survival

Journal of Cellular Physiology
Joseph LomakoKermit L Carraway

Abstract

Muc4/sialomucin complex (SMC), a large heterodimeric mucin composed of an extracellular mucin subunit ASGP-1 and a transmembrane subunit ASGP-2, is present at the rat ocular surface localized mainly to the most superficial layers of the epithelia. To investigate corneal homeostasis and the functions of Muc4/SMC at the ocular surface, we developed a corneal epithelial cell culture system from corneal explants, from which migrating cells formed an epithelial sheet resembling the native epithelium with regard to microanatomy, expression of characteristic markers, cell migration, and Muc4/SMC expression. Cells migrating from the explants expressed smooth muscle actin. Proliferation was detected only on the edge of epithelial sheet in the immature epithelium and throughout the sheet in confluent cultures. Microscopy revealed that the epithelial sheet was formed from four to six layers of cells expressing keratin 3 and Muc4/SMC in forms identical to those expressed at ocular surface in vivo. Electron microscopy showed cells in various morphological states in the process of releasing from the surface of the multilayer (desquamating). Surprisingly, few of these cells showed evidence of apoptosis, either by morphological or DNA fragment...Continue Reading

References

Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·G J Strous, J Dekker
Oct 23, 1997·Current Opinion in Cell Biology·S M Frisch, E Ruoslahti
Jan 31, 1998·The Journal of Biological Chemistry·M KomatsuK L Carraway
Oct 8, 1998·The Biochemical Journal·S A Price-SchiaviK L Carraway
Feb 20, 1999·The Journal of Biological Chemistry·K L CarrawayK L Carraway
Nov 26, 1999·Biology of Reproduction·N Idris, K L Carraway
May 23, 2000·Methods in Molecular Biology·K L Carraway
Feb 28, 2001·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·D T WeedK L Carraway
Feb 16, 2002·Experimental Eye Research·B A Dalton, J G Steele
Jan 1, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kermit L CarrawayCoralie A Carothers Carraway
May 27, 2003·Investigative Ophthalmology & Visual Science·Pablo ArgüesoIlene K Gipson

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Citations

Jan 11, 2007·Histochemistry and Cell Biology·Kristin JägerFriedrich P Paulsen
Apr 15, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Andreja ErmanKristijan Jezernik
Dec 16, 2006·Current Eye Research·Jin ZhangKermit L Carraway
Mar 13, 2010·Experimental Eye Research·Bharathi Govindarajan, Ilene K Gipson
Apr 24, 2009·Journal of Cellular Biochemistry·Wieslawa M LomakoKermit L Carraway
Jan 8, 2010·Journal of Cellular Physiology·Joseph LomakoKermit L Carraway
Jun 27, 2006·Progress in Histochemistry and Cytochemistry·Friedrich P Paulsen, Monica S Berry
Jan 23, 2013·International Journal of Molecular Medicine·Yevgeny TendlerGary Weisinger

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