PMID: 9445077Jan 28, 1998Paper

Non-AUG translation initiation in a plant RNA virus: a forty-amino-acid extension is added to the N terminus of the soil-borne wheat mosaic virus capsid protein

Journal of Virology
Yukio Shirako

Abstract

RNA 2 of soil-borne wheat mosaic virus (SBWMV), the type species of the genus Furovirus, encodes a protein previously hypothesized to be initiated at an in-frame non-AUG codon upstream of the AUG initiation codon (nucleotide positions 334 to 336) for the 19-kDa capsid protein. Site-directed mutagenesis and in vitro transcription and translation analysis indicated that CUG (nucleotides 214 to 216) is the initiation codon for a protein with a calculated molecular mass of 25 kDa composed of a 40-amino-acid extension to the N terminus of the 19-kDa capsid protein. A stable deletion mutant, which was isolated after extensive passages of a wild-type SBWMV, contained a mixture of two deleted RNA 2's, only one of which coded for the 25-kDa protein. The amino acid sequence of the N-terminal extension was moderately conserved and the CUG initiation codon was preserved among three SBWMV isolates from Japan and the United States. This amino acid sequence conservation, as well as the retention of expression of the 25-kDa protein in the stable deletion mutant, suggests that the 25-kDa protein is functional in the life cycle of SBWMV. This is the first report of a non-AUG translation initiation in a plant RNA virus genome.

References

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Citations

Sep 1, 2003·Molecular Plant Pathology·Konstantin KanyukaMichael J Adams
Sep 27, 2005·Plant Cell Reports·Annie DepeigesGeorges Picard
Mar 26, 2003·The Journal of General Virology·Shuichi OhsatoYukio Shirako
Oct 24, 2003·The Journal of General Virology·Hanbing AnJeanmarie Verchot-Lubicz
Nov 18, 2004·Journal of Cellular Biochemistry·Joseph A VetroYie-Hwa Chang
May 11, 2012·The Journal of General Virology·Anna Maria VairaJohn Hammond
Nov 24, 1999·Molecular and Cellular Biology·J L RiechmannE M Meyerowitz
May 21, 2021·Nature·Jessica Sook Yuin HoIvan Marazzi
Dec 21, 2005·Virology·Theo W Dreher, W Allen Miller

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