Non-biofilm-forming commensal Staphylococcus epidermidis isolates produce biofilm in the presence of trypsin

MicrobiologyOpen
Sergio Martínez-GarcíaJuan C Cancino-Diaz

Abstract

Epidemiological studies comparing clinical and commensal Staphylococcus epidermidis isolates suggest that biofilm formation is a discriminant biomarker. A study showed that four non-biofilm-forming clinical S. epidermidis isolates could form an induced biofilm by trypsin treatment, suggesting that S. epidermidis can form biofilms in a protease-independent way and in a trypsin-induced way. In this study, the trypsin capacity to induce biofilm formation was evaluated in non-biofilm-forming S. epidermidis isolates (n = 133) in order to support this mechanism and to establish the importance of total biofilms (meaning the sum of protease-independent biofilm and trypsin-induced biofilm). Staphylococcus epidermidis isolates from ocular infections (OI; n = 24), prosthetic joint infections (PJI; n = 64), and healthy skin (HS-1; n = 100) were screened for protease-independent biofilm formation according to Christensen's method. The result was that there are significant differences (p < .0001) between clinical (43.2%) and commensal (17%) protease-independent biofilm producers. Meanwhile, non-biofilm-forming isolates were treated with trypsin, and biofilm formation was evaluated by the same method. The number of commensal trypsin-induced b...Continue Reading

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Feb 2, 2019·The Journal of Microbiology·Sergio Martínez-GarcíaJuan C Cancino-Diaz

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Citations

Nov 27, 2020·Computational and Structural Biotechnology Journal·Hoai T T NguyenMichael Otto

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Methods Mentioned

BETA
electrophoresis
genotyping
scanning electron microscopy

Software Mentioned

BLAST
GraphPad Prism

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