Non-canonical Inflammasome-Mediated IL-1β Production by Primary Endometrial Epithelial and Stromal Fibroblast Cells Is NLRP3 and Caspase-4 Dependent

Frontiers in Immunology
Paul KellyCliona O'Farrelly

Abstract

Inflammation of the post-partum uterus is a normal physiological event, crucial for tissue involution and repair. However, in the bovine, some cows fail to resolve this inflammation, resulting in endometritis, which compromises fertility. Earlier work has identified upregulated expression of the potent inflammatory cytokine IL-1β early post-partum, in cows which subsequently develop endometritis. As a result, targeting IL-1β expression holds potential as a novel treatment for this disease, yet the regulatory mechanisms contributing to IL-1β expression in the bovine endometrium remain unknown. To investigate this, endometrial tissue samples were obtained 7 and 21 days post-partum (DPP) from cows that were diagnosed with endometritis at 21 DPP and cows that experienced a physiological level of inflammation throughout involution. IL-1β was measured by qPCR, ELISA, and immunohistochemistry. Seven DPP, endometrial IL-1β protein levels were significantly higher in animals that proceeded to develop endometritis at 21 DPP. IL-1β production could be detected in luminal and glandular epithelium, in underlying stromal fibroblasts as well as infiltrating immune cells. To investigate the mechanisms regulating IL-1β expression, primary endom...Continue Reading

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Citations

Jan 19, 2021·Medicinal Research Reviews·Carolina PellegriniVincenzo Calderone
Dec 29, 2020·Frontiers in Physiology·Zhenzhen SunZhanjun Jia
Jul 3, 2021·Antioxidants·Burak Ibrahim AriozSermin Genc
Oct 11, 2021·Journal of Molecular Biology·Madeline J ChurchillIsabella Rauch

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Methods Mentioned

BETA
RNA-seq
biopsies
ELISA
biopsy
flow cytometry
transfection
PCR
amplicon dissociation
Protein Assay
Assay

Software Mentioned

GeNorm
Primer BLAST
GraphPad Prism
FlowJo

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