PMID: 26328273Sep 4, 2015Paper

Non-caveolar caveolin-1 expression in prostate cancer cells promotes lymphangiogenesis

Oncoscience
Zeyad D NassarMarie-Odile Parat

Abstract

Lymphangiogenesis allows prostate cancer (PCa) lymphatic metastasis, which is associated with poor prognosis and short survival rates. Caveolin-1 (Cav-1) is a membrane protein localized in caveolae, but also exists in non-caveolar, cellular or extracellular forms. Cav-1 is overexpressed in PCa, promotes prostate tumour progression and metastasis. We investigated the effect of caveolar and non-caveolar Cav-1 on PCa lymphangiogenic potential. Cav-1 was down-regulated in PC3 and DU145, and ectopically expressed in LNCaP cells. The effect of PCa cell conditioned media on lymphatic endothelial cell (LEC) viability, chemotaxis, chemokinesis and differentiation was assessed. The effect of Cav-1 on PCa cell expression of lymphangiogenesis-modulators VEGF-A and VEGF-C was assessed using qPCR and ELISA of the conditioned medium. Non-caveolar Cav-1, whether exogenous or endogenous (in LNCaP and PC3 cells, respectively) enhanced LEC proliferation, migration and differentiation. In contrast, caveolar Cav-1 (in DU145 cells) did not significantly affect PCa cell lymphangiogenic potential. The effect of non-caveolar Cav-1 on LECs was mediated by increased expression of VEGF-A as demonstrated by neutralization by anti-VEGF-A antibody. This stud...Continue Reading

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Citations

Aug 1, 2019·Biomolecules·America CamposAndrew Frederick Geoffery Quest
May 3, 2020·Cancer Metastasis Reviews·Zeyad D Nassar, Marie-Odile Parat
Jul 13, 2017·The Journal of Biological Chemistry·Takashi HiramaGregory D Fairn
Oct 27, 2017·Experimental and Therapeutic Medicine·Hao ChengYitao Ding
May 28, 2019·Frontiers in Cell and Developmental Biology·Michael G SugiyamaCostin N Antonescu
Jul 17, 2020·Molecular Cancer Research : MCR·Zeyad D NassarLisa M Butler
May 9, 2021·International Journal of Experimental Pathology·Mahdieh AliyariFariborz Bahrehmand
Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bowen YangZhiyu Wang

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