Non-classical effects of prolactin on the innate immune response of bovine mammary epithelial cells: Implications during Staphylococcus aureus internalization

Microbial Pathogenesis
Ivan Medina-EstradaAlejandra Ochoa-Zarzosa

Abstract

Staphylococcus aureus has the ability to invade mammary epithelial cells (bMECs) causing mastitis. This event depends primarily on the α5β1 integrin in the host cell. In addition, bMECs are a target for the hormone prolactin (PRL), which can regulate β1 integrin-dependent actions related to differentiation and lactation. Previously, we demonstrated that bovine PRL (bPRL, 5 ng/ml) stimulates S. aureus internalization into bMECs. TLR2 is important during S. aureus infections, but its activation by PRL has not yet been established. The objective of this study was to determine the role of α5β1 integrin and TLR2 during S. aureus internalization into bMECs stimulated with bPRL. We demonstrated that the prolactin-stimulated internalization of S. aureus decreases in response to the blockage of α5β1 integrin (∼ 80%) and TLR2 (∼ 80%). bPRL increases the membrane abundance (MA) of α5β1 integrin (∼ 20%) and induces TLR2 MA (∼ 2-fold). S. aureus reduces the α5β1 integrin MA in bMECs treated with bPRL (∼ 75%) but induces TLR2 MA in bMECs (∼ 3-fold). Bacteria and bPRL did not modify TLR2 MA compared with the hormone alone. S. aureus induces the activation of the transcription factor AP-1, which was inhibited in bMECs treated with bPRL and inf...Continue Reading

References

May 7, 2005·Domestic Animal Endocrinology·K Svennersten-Sjaunja, K Olsson
Mar 21, 2006·The Veterinary Journal·Antonio ScibelliNorma Staiano
Apr 21, 2006·Research in Veterinary Science·José L Anaya-LópezAlejandra Ochoa-Zarzosa
Mar 27, 2007·Biochemical and Biophysical Research Communications·Agustina Rodriguez GranilloAlejandra Guberman
May 23, 2007·Molecular and Cellular Biology·K E SullivanM Bhatia
Apr 7, 2010·The International Journal of Biochemistry & Cell Biology·Tina Mele, Joaquín Madrenas
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Ronan KapetanovicMinou Adib-Conquy
May 13, 2011·European Journal of Cell Biology·Christine HoffmannChristof R Hauck
Aug 17, 2011·Microbial Pathogenesis·Leticia Lara-ZárateAlejandra Ochoa-Zarzosa
Oct 1, 2011·Veterinary Immunology and Immunopathology·Ynte H SchukkenUNKNOWN members of the Pfizer mastitis research consortium
Mar 8, 2013·Current Opinion in Microbiology·Kaiwen W Chen, Kate Schroder
Apr 11, 2013·International Reviews of Immunology·Miwa Sasai, Masahiro Yamamoto

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