Non-Coding RNA Pvt1 Promotes Cancer Stem Cell-Like Traits in Nasopharyngeal Cancer via Inhibiting miR-1207

Pathology Oncology Research : POR
Meng CuiSu-Xia Luo

Abstract

Nasopharyngeal carcinoma (NPC) is a kind of head-neck malignant tumor. lncRNA-PVT1 can promote the proliferation of carcinoma cells, and induce cells to have stem cell-like potentials. However, the function of PVT1 in NPC cells is not clear. The expressions of lncRNA-PVT1 and the expressions of the stem cell markers in NPC tissues or cell lines were investigated by qRT-PCR or western blot. The cell proliferation, and the ability of NPC cells to form spherical, clonal colonies were investigated by MTT assay, colony formation assay, and tumor-sphere formation assay. Cancer stem cells surface markers were detected by flow cytometry and western blot. PI3K/AKT signal activation in NPC cells was determined by western blot. PVT1 was significantly up-regulated in both NPC tissues and cell lines and associated with poor prognosis. PVT1 knockdown reduced NPC cells viability, clonogenicity, the cell surface CD44+/CD24- stem phenotype, and the expressions of the stem cell markers in NPC cells, including Oct4, c-Myc, SOX2, and ALDH. Furthermore, PVT1 negatively regulates the expression levels of miR-1207 in NPC cells and spheres cells, which is critical for NPC stemness. Knockdown of miR-1207 promoted stem phenotype and the expressions of t...Continue Reading

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Citations

Nov 30, 2019·Frontiers in Oncology·Ariadna BoloixMiguel F Segura
Aug 6, 2019·Frontiers in Oncology·Wenxi WangZhaoyang Zeng
Mar 3, 2020·Frontiers in Oncology·Onayemi Titilayo OnagoruwaOlorunseun O Ogunwobi
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Soudeh Ghafouri-FardGuive Sharifi
Sep 30, 2021·Expert Reviews in Molecular Medicine·Sepideh MirzaeiHaroon Khan

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Methods Mentioned

BETA
PCR
Flow Cytometry
protein assay
transfection

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