PMID: 11914103Mar 27, 2002Paper

Non-competitive immunochemiluminometric assay for cardiotrophin-1 detects elevated plasma levels in human heart failure

Clinical Science
Leong L NgSonja Jennings

Abstract

Cardiotrophin-1 (CT-1) leads to a specific form of ventricular hypertrophy characterized by sarcomeres added in series, and has been reported to be elevated in heart failure. Previous competitive assays for CT-1 necessitate the extraction of plasma and involve prolonged incubations. We describe the development of a non-competitive assay for CT-1 that can measure plasma levels without the need for extraction. Two antibodies specific for the mid-section (amino acids 105-120) and C-terminal (amino acids 186-199) portions of CT-1 were developed in rabbits. One antibody was immobilized and used as the capture antibody. The other antibody was affinity purified and biotinylated. Unextracted plasma was incubated with these antibodies, and detection was with methylacridinium ester-labelled streptavidin. Plasma was obtained from 40 patients with heart failure and 40 normal control subjects. The non-competitive assay demonstrated a linear increase in chemiluminescence (measured as relative light units) with increasing amounts of full-length recombinant CT-1, with no evidence of a hook effect at high concentrations. The lower limit of detection was 2.9 fmol/ml. Intra-assay coefficients of variation ranged from 3.1% to 4.2% in the 10-40 fmo...Continue Reading

Citations

Jan 26, 2007·European Journal of Clinical Pharmacology·S J GoldD G Lambert
Aug 23, 2003·Journal of the American College of Cardiology·Russell J O'BrienLeong L Ng
Oct 22, 2011·Clinical and Experimental Pharmacology & Physiology·Shan LiuHenry Krum
Jan 1, 2009·Mediators of Inflammation·Michael FritzenwangerHans-R Figulla
Dec 31, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Atac CelikUnal Erkorkmaz
Sep 17, 2008·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·David Stejskal, Viktor Ruzicka
Mar 13, 2012·Heart Failure Clinics·Viorel G Florea, Inder S Anand
Jul 20, 2007·Cytokine·Michael FritzenwangerHans-R Figulla
Jul 24, 2014·The Journal of Clinical Hypertension·Kangxing SongArya Mani
Oct 29, 2004·The EMBO Journal·Sylvain PradervandKenneth R Chien

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