Non-Covalent Associates of siRNAs and AuNPs Enveloped with Lipid Layer and Doped with Amphiphilic Peptide for Efficient siRNA Delivery

International Journal of Molecular Sciences
Julia PoletaevaInna Pyshnaya

Abstract

Elaboration of non-viral vehicles for delivery of therapeutic nucleic acids, in particular siRNA, into a cell is an actively growing field. Gold nanoparticles (AuNPs) occupy a noticeable place in these studies, and various nanoconstructions containing AuNPs are reported. We aimed our work to the rational design of AuNPs-based siRNA delivery vehicle with enhanced transfection efficiency. We optimized the obtaining of non-covalent siRNAs-AuNPs cores: ionic strength, temperature and reaction time were determined. Formation of cores was confirmed using gel electrophoresis. Stable associates were prepared, and then enveloped into a lipid layer composed of phosphatidylcholine, phosphatidylethanolamine and novel pH-sensitive lipidoid. The constructions were modified with [Str-(RL)₄G-NH₂] peptide (the resulting construction). All intermediate and resulting nanoconstructions were analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) to control their physico-chemical properties. To examine the biological effect of the delivery vehicle, green fluorescent protein (GFP)-expressing human embryonic kidney (HEK) Phoenix cells were incubated with the resulting construction containing anti-GFP siRNA, with the siRN...Continue Reading

References

Sep 19, 2002·Journal of the American Chemical Society·Linette M DemersChad A Mirkin
Sep 21, 2004·Biophysical Journal·Siewert-Jan Marrink, Alan E Mark
Mar 9, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Stefania Rapino, Francesco Zerbetto
Aug 9, 2005·Journal of Proteome Research·Amina S Woods, Sergi Ferré
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·L Bellon
Jun 26, 2008·BMC Molecular Biology·Cordula TschuchDaniel Mertens
Sep 27, 2008·Small·Won-Kyu RhimJwa-Min Nam
Jan 28, 2009·Journal of the American Chemical Society·David A GiljohannChad A Mirkin
Mar 5, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Akin AkincDaniel G Anderson
Feb 16, 2011·Nano Letters·Kaylin M McMahonC Shad Thaxton
May 7, 2011·Journal of the American Chemical Society·Jessica R MarksWilliam C Wimley
Jun 3, 2011·Journal of the American Chemical Society·Joshua I CutlerChad A Mirkin
Jun 21, 2014·Proceedings of the National Academy of Sciences of the United States of America·Stacey N BarnabyChad A Mirkin
Aug 15, 2014·Analytical Chemistry·Yongming GuoXingyu Jiang
Jul 21, 2015·Nature Biotechnology·Anders WittrupJudy Lieberman
Jul 28, 2016·International Journal of Nanomedicine·Timofei S ZatsepinVictor Koteliansky
Mar 1, 2017·Nature Biotechnology·Steven F Dowdy
Aug 2, 2017·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kaushik ThankiCamilla Foged
Aug 10, 2017·Applied Microbiology and Biotechnology·Chit TamTzi Bun Ng
Dec 13, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Anna EpanchintsevaInna Pyshnaya
Dec 16, 2017·Cancer Metastasis Reviews·Xiuhui ChenAnil K Sood
Jun 6, 2018·Chemical Communications : Chem Comm·N Basaran Mutlu AgardanVladimir P Torchilin
Jun 9, 2018·Journal of Biomedical Nanotechnology·Xi YangSunil Krishnan

❮ Previous
Next ❯

Citations

Jan 11, 2020·Beilstein Journal of Nanotechnology·Anna V EpanchintsevaInna A Pyshnaya
Aug 11, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Niyou WangA Senthil Kumar
Jul 2, 2020·International Journal of Nanomedicine·Yan WangQi Li
May 6, 2021·Nanomaterials·Anna V EpanchintsevaDmitrii V Pyshnyi
Jul 3, 2021·International Journal of Molecular Sciences·Ruslan KashapovOleg Sinyashin
May 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Pavel VorobjevDmitrii Pyshnyi
Sep 21, 2019·The Journal of Physical Chemistry. B·Ilya S DovydenkoMaxim N Sokolov

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
dynamic light scattering
confocal microscopy
transfection
column chromatography
transmission
transmission electron microscopy
flow cytometry

Software Mentioned

NovoExpress

Related Concepts

© 2022 Meta ULC. All rights reserved