Non-covalent binding of nucleic acids with gold nanoparticles provides their stability and effective desorption in environment mimicking biological media

Nanotechnology
Anna EpanchintsevaInna Pyshnaya

Abstract

Ability of gold nanoparticles to bind different substances determines high interest of researchers to their usage as a promising carrier of various biological substances including nucleic acids (NAs) for therapeutic applications. Most publications report covalent binding (conjugation) of a nucleic acid to spherical AuNPs via Au-S bond. In this work, we obtained non-covalent associates of different ssDNA, ssRNA and siRNAs with spherical gold nanoparticles (AuNPs) and examined their physico-chemical properties and stability in media mimicking intracellular space (bacterial "cytosol") and cell culture media (10% FBS in DMEM). The "cytosol" was obtained from E. coli and possessed nuclease activity. For the first time, we used phosphoryl guanidine (dimethylimidazolidin-2-imine, Dmi) group for modification of 3´-ends to enhance stability of ssRNAs and siRNAs against nucleases destruction.
 Trying to evaluate the material balance, we analyzed whole nucleotide species obtained after incubation of NA-AuNPs associates in "cytosol" and FBS and evaluated degree of NAs destruction, a share of remained full-size NAs on surface of AuNPs and in solution. Native ss- and siRNAs, both free and in composition of non-covalent associates with Au...Continue Reading

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Citations

Jan 11, 2020·Beilstein Journal of Nanotechnology·Anna V EpanchintsevaInna A Pyshnaya
Oct 30, 2020·Diagnostics·Alexey S ChubarovDmitrii V Pyshnyi
May 6, 2021·Nanomaterials·Anna V EpanchintsevaDmitrii V Pyshnyi
May 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Pavel VorobjevDmitrii Pyshnyi

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