Non-destructive measurement of chlorophyll b : a ratios and identification of photosynthetic pathways in grasses by reflectance spectroscopy

Functional Plant Biology : FPB
Katharina Siebke, Marilyn C Ball

Abstract

Equations for non-destructive determination of chlorophyll b : a ratios in grasses were developed from reflectance spectra of intact leaves of barley (Hordeum vulgare L.) and two barley mutants: clorina f2, which lacks chlorophyll b and clorina f104, which has a low chlorophyll b content. These plants enabled separation of effects of chlorophyll composition on reflectance spectra due to differential light absorption by chlorophylls a and b and to measure the effects of chlorophyll b on the contribution of fluorescence emitted by chlorophyll a to the reflectance spectra. Indices developed from these data were then tested on growth chamber-grown leaves from six C3 and 17 C4 grass species (7 NAD-ME and 10 NADP-ME subtypes). We used the chlorophyll b : a ratio because the data were less skewed than the chlorophyll a : b ratio. The best index for determination of the chlorophyll b : a ratio utilised wavelengths affected by chlorophyll absorbance: [R626 - 0.5 (R603 + R647)]/[R552- R626]. The chlorophyll b : a ratio was significantly lower in the C4 than C3 grasses, but was not sufficient in itself to separate these two functional groups. However, because of differences in fluorescence characteristics, C3 and C4 species could be disti...Continue Reading

References

Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A M Gilmor Govindjee
Apr 13, 2002·Photochemistry and Photobiology·Anatoly A GitelsonMark N Merzlyak
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·B. R. Green, D. G. Durnford
Jul 31, 2007·Plant, Cell & Environment·Rowan F Sage, David S Kubien

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Citations

Jun 2, 2016·Nature Plants·Walter JetzSusan L Ustin
Jul 11, 2018·Plant Methods·Leandro do Prado RibeiroChristian Nansen
Jul 8, 2010·Journal of Experimental Botany·Rana MunnsHamlyn G Jones
Jan 18, 2017·Plant, Cell & Environment·José Ignacio García-PlazaolaVíctor Resco de Dios

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