Non-DNA-binding antibodies in patients with lupus nephritis could recognize membrane proteins of glomerular mesangial cells.

Journal of Clinical Immunology
Hui DuMing-Hui Zhao

Abstract

Lupus nephritis (LN) is a prototypic autoimmune disease, however, the precise immuno-pathogenesis of LN remains to be elucidated. In our previous studies, autoantibodies against mesangial cells had been identified in sera from patients with lupus nephritis and could bind the membrane proteins of human mesangial cells (HMC) directly through antigen-antibody interaction without DNA bridge. The current study is to investigate whether the autoantibodies were associated with anti-DNA antibodies and their target antigens distribution in different cell types. Sera from nine patients with renal biopsy proven lupus nephritis with positive anti-dsDNA antibodies and four healthy subjects were collected. IgG was isolated by Protein G affinity chromatography and then non-DNA-binding IgG fractions were obtained after deletion of anti-DNA antibodies using a DNA-cellulose affinity column. Membrane proteins, obtained from HMC, human umbilical vein endothelial cells (HUVEC), peripheral mononuclear cells by sonication and sequential centrifugation, were solubilized and applied in Western-blot analysis to characterize the target antigens. In results, the non-DNA-binding IgG fractions from sera of patients with lupus nephritis could blot the protei...Continue Reading

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Citations

Apr 24, 2010·Clinical Reviews in Allergy & Immunology·Catherine Hanrotel-SaliouYves Renaudineau
May 21, 2020·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Jeff Chien-Fu LinWen-Miin Liang
May 25, 2010·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Jenson C S Mak, Steven Faux
Jun 14, 2014·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Qingxi MengTingbao Zhao
Sep 7, 2014·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Jun ZhouXin Guo
Mar 2, 2012·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Jun ZhouChengqi He

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