PMID: 6170344Sep 29, 1981Paper

Non-enzymatic acetylation of human hemoglobins

Biochimica Et Biophysica Acta
G J Garbutt, E C Abraham

Abstract

Chromatographically purified Hb F0, Hb A0 and Hb S0 fractions were incubated with 70 microM [14C]acetyl-CoA for 3 h in 20 mM Tris-HCl, pH 7.6 and 8.6. The acid-precipitable radioactivity was monitored and the hemoglobins were separated by Biorex 70 chromatography. The pH dependence of acetylation showed an increase in acetylation with an increase in pH from 7.6 to 8.6, whereas an increase in ionic strength decreased acetylation. Incubation of Hb F0 with [14C]acetyl-CoA resulted in modified hemoglobin and gamma chains that co-chromatographed with Hb FIc and gamma 1c chains, respectively. Acetylation of Hb A0 and Hb S0 produced minor hemoglobins whose chromatographic mobilities were slightly faster than those of Hb AIc and Hb SIc, respectively, Radioactivity peaks also appeared at the leading edges of the major hemoglobin zones as well, which indicates that, like non-enzymatic glycosylation, non-enzymatic acetylation of hemoglobins involves both specific and nonspecific reactions.

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Citations

Jan 1, 1985·CRC Critical Reviews in Biochemistry·H P DriessenH Bloemendal
Jun 21, 2012·Biochemical and Biophysical Research Communications·Mattia PolettoGianluca Tell
Jun 18, 2015·ACS Chemical Biology·Adam S OliaRonen Marmorstein
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Jan 1, 1988·Life Sciences·C C QiuE C Abraham
Oct 15, 1984·Life Sciences·J Kasten-Jolly, F Taketa

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