Non-esterified fatty acids impair endothelium-dependent vasodilation in rat mesenteric resistance vessels

Clinical Science
Christopher A R SainsburyJohn R Petrie

Abstract

Elevated circulating levels of NEFAs (non-esterified fatty acids) are associated with states of insulin resistance and increased risk of vascular disease. Previous animal and human studies have demonstrated NEFA-induced endothelial dysfunction of large conduit arteries, reversible by the antioxidant ascorbic acid. We therefore investigated the effect of NEFAs on carbachol-induced endothelium-dependent vasodilation of rat resistance arteries in vitro using the technique of wire myography. In addition, we investigated the effect of co-incubation of NEFAs and ascorbic acid. Cumulative concentration-response curves to carbachol (endothelium-dependent vasodilation) and SNAP (S-nitroso-N-acetyl-DL-penicillamine; endothelium-independent vasodilation) were constructed. Those to carbachol were repeated following a 30 min incubation with either oleic acid (10(-4) M) or palmitic acid (10(-4) M), demonstrating significant impairment of endothelium-dependent vasodilation with both [P<0.05, comparison of pD2 values (the negative log concentration of agonist required to effect a 50% response)]. A cumulative concentration-response curve to carbachol was repeated following co-incubation with palmitic acid (10(-4) M) and the antioxidant ascorbic...Continue Reading

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Citations

Oct 11, 2012·The British Journal of Nutrition·Ana Rodriguez-MateosJunji Terao
May 17, 2006·Clinical and Experimental Pharmacology & Physiology·David W Stepp
Jun 24, 2011·The American Journal of Clinical Nutrition·Jason H Y WuDariush Mozaffarian
Aug 19, 2009·Archives of Physiology and Biochemistry·Giovanni AnfossiMariella Trovati
Nov 6, 2012·Microvascular Research·Jill J F BelchFaisel Khan
Jan 10, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Rany Vorn, Hae Young Yoo
Sep 18, 2015·Nutrients·Maharshi BhaswantLindsay Brown
Mar 10, 2006·Clinical Science·Indika EdirisingheC Tissa Kappagoda
Sep 10, 2019·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Rany Vorn, Hae Young Yoo
Jul 1, 2020·Critical Reviews in Food Science and Nutrition·Gengdong ChenZheqing Zhang
Jan 4, 2021·Aging Clinical and Experimental Research·Antonio Capurso, Cristiano Capurso

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