Non-homologous DNA end joining and alternative pathways to double-strand break repair

Nature Reviews. Molecular Cell Biology
Howard H Y ChangMichael R Lieber

Abstract

DNA double-strand breaks (DSBs) are the most dangerous type of DNA damage because they can result in the loss of large chromosomal regions. In all mammalian cells, DSBs that occur throughout the cell cycle are repaired predominantly by the non-homologous DNA end joining (NHEJ) pathway. Defects in NHEJ result in sensitivity to ionizing radiation and the ablation of lymphocytes. The NHEJ pathway utilizes proteins that recognize, resect, polymerize and ligate the DNA ends in a flexible manner. This flexibility permits NHEJ to function on a wide range of DNA-end configurations, with the resulting repaired DNA junctions often containing mutations. In this Review, we discuss the most recent findings regarding the relative involvement of the different NHEJ proteins in the repair of various DNA-end configurations. We also discuss the shunting of DNA-end repair to the auxiliary pathways of alternative end joining (a-EJ) or single-strand annealing (SSA) and the relevance of these different pathways to human disease.

References

Dec 1, 1992·Current Opinion in Biotechnology·R R Sinden, R D Wells
Sep 5, 1989·Biochemistry·M J BlommersC W Hilbers
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M R LieberM Gellert
Jan 1, 1996·Molecular and Cellular Biology·G H Gauss, M R Lieber
Sep 19, 1998·Molecular and Cellular Biology·R B WestM R Lieber
Feb 3, 1999·Annual Review of Genetics·J E Haber
Apr 11, 2000·Molecular and Cellular Biology·S A Nick McElhinnyD A Ramsden
Nov 10, 2001·Nature Structural Biology·B L SibandaL Pellegrini
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lanying LiMorton J Cowan
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Y DaiP A Jeggo
Mar 18, 2003·Molecular and Cellular Biology·Stephanie A Nick McElhinny, Dale A Ramsden
Aug 13, 2003·The Journal of Biological Chemistry·Patrick SungMichael G Sehorn
Aug 30, 2003·Oncogene·Kristoffer Valerie, Lawrence F Povirk
Jan 7, 2004·Nature Reviews. Molecular Cell Biology·Michael R Lieber, Zarir E Karanjawala
Sep 24, 2004·The EMBO Journal·Christine Anne KochDaniel Durocher
Dec 3, 2004·Molecular Cell·Yunmei MaMichael R Lieber
Jan 20, 2005·The Journal of Experimental Medicine·Qiang Pan-HammarströmMichael R Ehrenstein
Jan 20, 2005·Molecular and Cellular Biology·James M Daley, Thomas E Wilson
Mar 8, 2005·Molecular Cell·Nina K BernsteinJ N Mark Glover
Jun 21, 2005·The Journal of Biological Chemistry·James M DaleyThomas E Wilson
Aug 12, 2005·The Journal of Biological Chemistry·Yunmei MaMichael R Lieber
Nov 16, 2005·Annual Review of Genetics·James M DaleyThomas E Wilson
Dec 6, 2005·Nature Cell Biology·Ali JazayeriStephen P Jackson
Jun 24, 2006·Methods in Enzymology·Xi ChenAlan E Tomkinson
Jul 25, 2006·Immunity·Michael R Lieber
Jul 29, 2006·The EMBO Journal·Aaron A GoodarziSusan P Lees-Miller
Aug 18, 2006·The Journal of Biological Chemistry·Doris NiewolikKlaus Schwarz
Nov 24, 2006·The Journal of Biological Chemistry·Lawrence F PovirkSteven M Yannone
Dec 13, 2006·Nature Structural & Molecular Biology·Andrea F MoonLars C Pedersen
Feb 10, 2007·The EMBO Journal·Jiafeng GuMichael R Lieber
Mar 14, 2007·Molecular and Cellular Biology·Natasha IlesKeith W Caldecott

❮ Previous
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Citations

Feb 20, 2018·Cell Biology International·Kelsey P RingerJonathan K Alder
Feb 18, 2018·The FEBS Journal·Jay H Chung
Nov 8, 2017·International Journal of Molecular Sciences·Luciana R GomesGiovana S Leandro
Feb 9, 2018·International Journal of Molecular Sciences·Kez ClealDuncan Baird
Feb 2, 2018·Viruses·Makda GebreBenjamin E Gewurz
Nov 29, 2017·Nucleic Acids Research·Jakub A KochanPrzemek M Krawczyk
Feb 8, 2018·Journal of Cellular and Molecular Medicine·Jicong DuCong Liu
Apr 28, 2018·Nature Reviews. Molecular Cell Biology·Eytan Zlotorynski
Nov 17, 2017·Blood·Hui-Ling Ou, Björn Schumacher
May 2, 2018·Genome Research·Robert KoflerChristian Schlötterer
Mar 14, 2018·The Journal of Biological Chemistry·Annahita Sallmyr, Alan E Tomkinson
Dec 17, 2017·The Journal of Biological Chemistry·Nicholas R PannunzioMichael R Lieber
Aug 1, 2018·Blood Advances·David BuchbinderRoshini S Abraham
Sep 14, 2018·Bioscience Reports·Andrew CubbonEdward L Bolt
Oct 5, 2018·Oncology Research and Treatment·Anna SchmittH Christian Reinhardt
Oct 19, 2017·F1000Research·Raghavendra A ShamannaVilhelm A Bohr
Oct 10, 2018·Molecular Carcinogenesis·Vidya GopalakrishnanBibha Choudhary
Jan 11, 2019·Cells·Camille LaberthonnièreJérôme D Robin
Mar 6, 2019·Journal of Cellular Biochemistry·Fengyan LiuShili Liu
Mar 14, 2019·Haemophilia : the Official Journal of the World Federation of Hemophilia·Xiaoling XieQiulan Ding
Feb 13, 2019·Molecular Microbiology·Claire BertrandPierre Leblond
Sep 20, 2017·Cell Research·Go WatanabeDewight Williams
Jul 12, 2017·Nature Communications·Shinta SaitoNoritaka Adachi
Feb 10, 2018·Current Medicinal Chemistry·Monika TomaTomasz Sliwinski
May 22, 2019·Diagnostics·Stergios BoussiosNicholas Pavlidis
Jul 23, 2019·The FEBS Journal·Aya KurosawaNoritaka Adachi
Jul 3, 2019·Nature Reviews. Molecular Cell Biology·Ralph ScullyNicholas A Willis
Jul 17, 2019·Nature Plants·Yingxiao ZhangYiping Qi

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