PMID: 11925397Apr 2, 2002Paper

Non-invasive amino acid turnover predicts human embryo developmental capacity

Human Reproduction
F D HoughtonHenry J Leese

Abstract

IVF is limited by low success rates and a confounding high multiple birth rate contributing to prematurity, increased neonatal mortality and child handicap. These problems could be overcome if single embryos of known developmental competence could be selected for transfer on day 2/3 of development, but current methods, which rely on morphological appearance, are poor predictors of viability. We have measured non-invasively the depletion/appearance (i.e. turnover) of a physiological mixture of 18 amino acids by single human embryos during in-vitro culture using high performance liquid chromatography. From the time of transfer (day 2/3), embryos with future competence to develop to the blastocyst stage (day 5/6) exhibit amino acid flux patterns distinct from those of embryos with similar morphological appearance which arrest. Significantly, the profiles of Ala, Arg, Gln, Met and Asn flux predict blastocyst potentiality at >95%. The amino acid most consistently depleted throughout development by those embryos which form blastocysts was leucine. Of the amino acids which were produced, the most striking was alanine, which appeared in increasing amounts throughout development. Non-invasive amino acid profiling has the potential to se...Continue Reading

Citations

Sep 5, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Henry J Leese
Dec 14, 2006·Journal of Assisted Reproduction and Genetics·Lars D M OttosenHans Jakob Ingerslev
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