Non-invasive Chamber-Specific Identification of Cardiomyocytes in Differentiating Pluripotent Stem Cells

Stem Cell Reports
Eva BrauchleKatja Schenke-Layland

Abstract

One major obstacle to the application of stem cell-derived cardiomyocytes (CMs) for disease modeling and clinical therapies is the inability to identify the developmental stage of these cells without the need for genetic manipulation or utilization of exogenous markers. In this study, we demonstrate that Raman microspectroscopy can non-invasively identify embryonic stem cell (ESC)-derived chamber-specific CMs and monitor cell maturation. Using this marker-free approach, Raman peaks were identified for atrial and ventricular CMs, ESCs were successfully discriminated from their cardiac derivatives, a distinct phenotypic spectrum for ESC-derived CMs was confirmed, and unique spectral differences between fetal versus adult CMs were detected. The real-time identification and characterization of CMs, their progenitors, and subpopulations by Raman microspectroscopy strongly correlated to the phenotypical features of these cells. Due to its high molecular resolution, Raman microspectroscopy offers distinct analytical characterization for differentiating cardiovascular cell populations.

References

Mar 1, 1988·Biophysical Journal·M PézoletJ P Caillé
Feb 19, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P S ZammitM E Buckingham
Jun 1, 2002·Circulation Research·Kishore B S Pasumarthi, Loren J Field
May 22, 2003·Cardiovascular Research·S D BirdC L Mummery
Apr 12, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Christoph KrafftReiner Salzer
Jan 19, 2007·Nature Clinical Practice. Cardiovascular Medicine·Susan ChungAndre Terzic
Apr 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Irit HuberLior Gepstein
May 23, 2007·Heart Failure Reviews·Mitra RajabiHeinrich Taegtmeyer
Jun 15, 2007·Heart·Jonathan LeorJoseph Itskovitz-Eldor
Sep 30, 2009·Circulation·Limor ZwiLior Gepstein
Feb 2, 2013·Analytical and Bioanalytical Chemistry·Sebastian DochowJürgen Popp
May 2, 2013·American Journal of Medical Genetics. Part a·Marc SylvaAntoon F M Moorman
Dec 11, 2013·Stem Cells Translational Medicine·Ioannis KarakikesRoger J Hajjar
Apr 8, 2014·Trends in Biotechnology·J Sulé-SusoG D Sockalingum
Apr 16, 2014·Scientific Reports·Eva BrauchleKatja Schenke-Layland
Jun 6, 2014·Science Translational Medicine·Elena MatsaJoseph C Wu
Oct 23, 2014·Nature Communications·Ralf GilsbachLutz Hein

❮ Previous
Next ❯

Citations

Sep 24, 2016·Biotechnology Journal·Eva Brauchle, Katja Schenke-Layland
Apr 6, 2018·Science Translational Medicine·Harsha D Devalla, Robert Passier
Aug 8, 2018·Acta Histochemica Et Cytochemica·Yasuaki KumamotoHideo Tanaka
Jul 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chia-Chen HsuHua Ye
Jul 21, 2021·International Journal of Molecular Sciences·Agata PrussBarbara Dołęgowska
Aug 28, 2018·Bioconjugate Chemistry·Ji Sun ChoiBrendan A C Harley
Dec 19, 2021·Analytical Chemistry·Dana Cialla-MayJürgen Popp

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA
fluorescence microscopy
fluorescence imaging
dissections

Software Mentioned

OriginPro
UnscramblerX
OPUS

Related Concepts

Related Feeds

Atrial Filbrillation

Atrial fibrillation refers to the abnormal heart rhythm characterized by rapid and irregular beating of the atria. Here is the latest research.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

© 2022 Meta ULC. All rights reserved