Non-invasive gene-expression-based detection of well-developed collateral function in individuals with and without coronary artery disease

Heart
P MeierC Seiler

Abstract

In patients with coronary artery disease (CAD), a well grown collateral circulation has been shown to be important. The aim of this prospective study using peripheral blood monocytes was to identify marker genes for an extensively grown coronary collateral circulation. Collateral flow index (CFI) was obtained invasively by angioplasty pressure sensor guidewire in 160 individuals (110 patients with CAD, and 50 individuals without CAD). RNA was extracted from monocytes followed by microarray-based gene-expression analysis. 76 selected genes were analysed by real-time polymerase chain reaction (PCR). A receiver operating characteristics analysis based on differential gene expression was then performed to separate individuals with poor (CFI<0.21) and well-developed collaterals (CFI>or=0.21) Thereafter, the influence of the chemokine MCP-1 on the expression of six selected genes was tested by PCR. The expression of 203 genes significantly correlated with CFI (p = 0.000002-0.00267) in patients with CAD and 56 genes in individuals without CAD (p = 00079-0.0430). Biological pathway analysis revealed 76 of those genes belonging to four different pathways: angiogenesis, integrin-, platelet-derived growth factor-, and transforming growth ...Continue Reading

Citations

Jun 6, 2013·BMC Medicine·Pascal MeierChristian Seiler
Mar 14, 2012·Journal of Clinical Bioinformatics·Rizwan MasudIftikhar J Kullo
Mar 20, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Joanna Głogowska-Ligus, Józefa Dąbek
Jan 3, 2012·Journal of Molecular and Cellular Cardiology·William M ChilianLiya Yin
Oct 1, 2011·Journal of Molecular and Cellular Cardiology·Paul F A TeunissenNiels van Royen
May 15, 2009·Journal of Cellular and Molecular Medicine·Sebastian GrundmannImo E Hoefer
Mar 6, 2013·Catheterization and Cardiovascular Interventions : Official Journal of the Society for Cardiac Angiography & Interventions·Wassef KarrowniKanu Chatterjee
Jun 11, 2010·European Journal of Clinical Investigation·Christian Seiler
Feb 2, 2010·Journal of the American College of Cardiology·Niels van RoyenWilliam F Fulton
May 10, 2016·F1000Research·Darawan RinchaiDamien Chaussabel

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