Non-invasive Imaging and Modeling of Liver Regeneration After Partial Hepatectomy

Frontiers in Physiology
Sara ZafarniaWiltrud Lederle

Abstract

The liver has a unique regenerative capability upon injury or partial resection. The regeneration process comprises a complex interplay between parenchymal and non-parenchymal cells and is tightly regulated at different scales. Thus, we investigated liver regeneration using multi-scale methods by combining non-invasive imaging with immunohistochemical analyses. In this context, non-invasive imaging can provide quantitative data of processes involved in liver regeneration at organ and body scale. We quantitatively measured liver volume recovery after 70% partial hepatectomy (PHx) by micro computed tomography (μCT) and investigated changes in the density of CD68+ macrophages by fluorescence-mediated tomography (FMT) combined with μCT using a newly developed near-infrared fluorescent probe. In addition, angiogenesis and tissue-resident macrophages were analyzed by immunohistochemistry. Based on the results, a model describing liver regeneration and the interactions between different cell types was established. In vivo analysis of liver volume regeneration over 21 days after PHx by μCT imaging demonstrated that the liver volume rapidly increased after PHx reaching a maximum at day 14 and normalizing until day 21. An increase in CD6...Continue Reading

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Citations

Jan 16, 2020·Regenerative Medicine Research·Feng ZhangShizhong Zheng
Sep 1, 2020·Archives of Toxicology·Abdel-Latif Seddek, Reham Hassan
Oct 16, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Ning HeXiaodong Jin
Oct 24, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Juan-Li DuanLin Wang

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Methods Mentioned

BETA
Protein Assay
Fluorescence

Software Mentioned

ExMI
GraphPad
- IT )
Gremse
AxioVisionRel
Prism
Imalytics Preclinical
ImageJ

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