Non-invasive longitudinal monitoring of angiogenesis in a murine full-thickness cutaneous wound healing model using high-resolution three-dimensional ultrasound imaging

Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
Kanae MukaiToshio Nakatani

Abstract

The aim of this study was to evaluate the longitudinal monitoring of angiogenesis in a murine full-thickness cutaneous wound healing model using high-resolution three-dimensional (3D) ultrasound imaging. Two C57BL/6 mice were used. Two-dimensional (2D) ultrasound images with the Color Doppler mode were acquired at regular spatial intervals on day 9, 11, and 14 after wounding. 3D ultrasound images were processed by reconstructing the 2D ultrasound image sequences. The wounds were harvested on day 14 and serial sections were immunohistologically stained with an anti-CD31 antibody. 3D ultrasound imaging with the Color Doppler mode showed the distribution of microvascular growth on days 9, 11, and 14 after wounding (44.6%, 51.5%, and 27.3% in wound 1, 55.8%, 38.1%, and 35.1% in wound 2, 60.6%, 62.6%, and 63.1% in wound 3, and 15.8%, 42.0%, and 31.9% in wound 4, respectively). A correlation was observed between % vascularity measured by paired 2D ultrasound imaging with the Color Doppler mode and sections immunohistologically stained for the anti-CD31 antibody (r=0.927, 0.871, 0.717, and 0.913 for wounds 1, 2, 3, and 4, respectively. P<.01). These results indicate that high-resolution 3D ultrasound imaging is useful for longitudinal...Continue Reading

References

Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·M G TonnesenR A Clark
Oct 10, 2006·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·C J StewartR C Bray
May 4, 2007·The Journal of Clinical Investigation·Harold Brem, Marjana Tomic-Canic
Oct 25, 2007·Journal of Ultrasound in Medicine : Official Journal of the American Institute of Ultrasound in Medicine·Andrej LyshchikJohn C Gore
Jun 6, 2009·Cardiovascular Research·Beat A Kaufmann
Sep 4, 2009·Journal of Cell Science·Tanya J Shaw, Paul Martin
Feb 9, 2010·Journal of Dental Research·S Guo, L A Dipietro
Aug 13, 2010·Developments in Ophthalmology·Martin J SiemerinkReinier O Schlingemann
Feb 24, 2011·Investigative Radiology·Christopher R AndersonJoshua J Rychak
May 20, 2011·Nature·Peter Carmeliet, Rakesh K Jain
Nov 24, 2012·International Wound Journal·Takuya HigashinoHiromi Sanada
Jul 12, 2013·PloS One·Ciceron O YanezKevin D Belfield
Nov 23, 2013·The American Journal of Pathology·Josef EhlingTwan Lammers
Oct 4, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Seunghyung LeeLawrence E Goldfinger
Apr 4, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Adelina CurajFabian Kiessling
Jul 15, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Mateusz S WietechaLuisa A DiPietro
Mar 25, 2016·Microvascular Research·Norifumi UraoLuisa A DiPietro

❮ Previous
Next ❯

Citations

Jun 16, 2021·Advances in Wound Care·Maximillian A WeigeltIra M Herman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Ahmad Y SheikhThomas K Hunt
Wounds : a Compendium of Clinical Research and Practice
Yukari NakajimaToshio Nakatani
Ultrasound in Obstetrics & Gynecology : the Official Journal of the International Society of Ultrasound in Obstetrics and Gynecology
F A GerardsJ M G van Vugt
Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
Y FerraqS Mordon
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Kimi ShimamuraMayumi Okuwa
© 2022 Meta ULC. All rights reserved