Non-invasive magnetic resonance imaging of oils in Botryococcus braunii green algae: Chemical shift selective and diffusion-weighted imaging

PloS One
Remco van SchadewijkA Alia

Abstract

Botryococcus braunii is an oleaginous green algae with the distinctive property of accumulating high quantities of hydrocarbons per dry weight in its colonies. Large variation in colony structure exists, yet its implications and influence of oil distribution and diffusion dynamics are not known and could not be answered due to lack of suitable in vivo methods. This publication seeks to further the understanding on oil dynamics, by investigating naturally relevant large (700-1500μm) and extra-large (1500-2500μm) sized colonies of Botryococcus braunii (race B, strain Showa) in vivo, using a comprehensive approach of chemical shift selective imaging, chemical shift imaging and spin echo diffusion measurements at high magnetic field (17.6T). Hydrocarbon distribution in large colonies was found to be localised in two concentric oil layers with different thickness and concentration. Extra-large colonies were highly unstructured and oil was spread throughout colonies, but with large local variations. Interestingly, fluid channels were observed in extra-large colonies. Diffusion-weighted MRI revealed a strong correlation between colony heterogeneity, oil distribution, and diffusion dynamics in different parts of Botryococcus colonies. ...Continue Reading

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Citations

Sep 25, 2020·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Catherine M KirklandMerle K de Kreuk

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Methods Mentioned

BETA
NMR
Chemical

Software Mentioned

Adobe Photoshop CC
Linux
CentOS
Meshmixer
Image Sequence Analysis tool package ( ISA )
Paravision
OriginLab
CSI
Tool
Bruker CSI Visualisation Tool

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