Non-invasive mass transfer measurements in complex biofilm-coated structures

Biotechnology and Bioengineering
D A Graf von der SchulenburgM L Johns

Abstract

We demonstrate a novel application of (13)C pulsed field gradient (PFG) NMR to monitor mass transfer, due to both flow and diffusion, in a 3D complex porous support structure modified by biofilm growth. This enables timescales an order of magnitude larger than previously possible to be accessed with respect to displacement probability distribution (propagator) measurements. The evolution in the propagator shape with observation time to the Gaussian asymptote (constant dispersion coefficient) is consequently well resolved. We also simulated the measured displacement propagators with good agreement between experiment and prediction. The methodology has significant potential for the selective characterization of the transport of nutrients, metabolic products, pollutants and biocides in such complex biofilm-containing structures.

References

Jul 1, 1980·Physics in Medicine and Biology·W A EdelsteinT Redpath
Oct 1, 1996·Journal of Magnetic Resonance. Series B·K PotterE W McFarland
Jul 2, 1999·Journal of Magnetic Resonance·B C HoskinsG Georgiou
Dec 11, 2002·Kidney International·Naomi BalabanJohan R Boelaert
Nov 11, 2003·Journal of Microbiological Methods·Scott ForsterJonathan Porter
Feb 6, 2004·Environmental Microbiology·Braden C DunsmoreHilary M Lappin-Scott
Mar 26, 2004·Journal of Magnetic Resonance·Joseph D SeymourPhilip S Stewart
Jul 21, 2004·Journal of Magnetic Resonance·Melanie M BrittonKen J Packer
Dec 17, 2004·Physical Review Letters·Joseph D SeymourRobin Gerlach
Feb 27, 2007·Journal of Magnetic Resonance·Belinda S AkpaLynn F Gladden
Jun 6, 2007·Water Science and Technology : a Journal of the International Association on Water Pollution Research·D A Graf von der SchulenburgM L Johns
Sep 20, 2007·Biotechnology and Bioengineering·D A Graf von der SchulenburgM L Johns

❮ Previous
Next ❯

Citations

Dec 24, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·J MitchellE J Fordham
Dec 22, 2012·Environmental Science & Technology·Alexis B SanderlinSarah L Codd
May 12, 2012·Computerized Medical Imaging and Graphics : the Official Journal of the Computerized Medical Imaging Society·Kelvin K L WongDerek Abbott
Aug 31, 2010·Journal of Contaminant Hydrology·Einar O FridjonssonSarah L Codd
Feb 26, 2010·FEMS Microbiology Ecology·Thomas R NeuJohn R Lawrence
Dec 16, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·A SartiE Foresti

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.