Non-invasive monitoring of in vivo hydrogel degradation and cartilage regeneration by multiparametric MR imaging

Theranostics
Zelong ChenRui Guo

Abstract

Numerous biodegradable hydrogels for cartilage regeneration have been widely used in the field of tissue engineering. However, to non-invasively monitor hydrogel degradation and efficiently evaluate cartilage restoration in situ is still challenging. Methods: A ultrasmall superparamagnetic iron oxide (USPIO)-labeled cellulose nanocrystal (CNC)/silk fibroin (SF)-blended hydrogel system was developed to monitor hydrogel degradation during cartilage regeneration. The physicochemical characterization and biocompatibility of the hydrogel were evaluated in vitro. The in vivo hydrogel degradation and cartilage regeneration of different implants were assessed using multiparametric magnetic resonance imaging (MRI) and further confirmed by histological analysis in a rabbit cartilage defect model for 3 months. Results: USPIO-labeled hydrogels showed sufficient MR contrast enhancement and retained stability without loss of the relaxation rate. Neither the mechanical properties of the hydrogels nor the proliferation of bone-marrow mesenchymal stem cells (BMSCs) were affected by USPIO labeling in vitro. CNC/SF hydrogels with BMSCs degraded more quickly than the acellular hydrogels as reflected by the MR relaxation rate trends in vivo. The mo...Continue Reading

Citations

Sep 6, 2018·Pharmaceutics·Sérgio R S VelosoElisabete M S Castanheira
Jul 23, 2019·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Cuijun DengYu Cheng
Aug 11, 2020·Biomaterials Science·David B BerrySamuel R Ward
Feb 18, 2020·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Seok Jung KimSang Heon Lee
Jul 19, 2019·Micromachines·Carmen J GilVahid Serpooshan
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Aug 23, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Taylor B LawsonMark W Grinstaff
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Methods Mentioned

BETA
chemical exchange
transmission electron microscopy
scanning electron microscopy
infrared spectroscopy

Software Mentioned

Imalytics Preclinical
SPSS

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