Non-invasive optical imaging of cathepsin B with activatable fluorogenic nanoprobes in various metastatic models

Biomaterials
Ju Hee RyuKwangmeyung Kim

Abstract

An increasing number of treatments of metastases rely on diagnostics and imaging these days. The facts that the activity of cathepsin B (CB) is markedly linked to the metastatic process and that CB is found highly expressed in the pericellular regions in this process make CB an attractive target for diagnosing metastases. We have developed a CB-sensitive nanoprobe (CB-CNP) consisting of self-quenched CB-sensitive fluorogenic peptide probes conjugated onto the surface of tumor-targeting glycol chitosan nanoparticles (CNPs). The freshly prepared CB-CNP formed a spherical nanoparticle structure (280 nm in diameter) and the fluorescence intensity of CB-CNP was strongly quenched in physiological condition. However, self-quenched CB-CNP boosted strong fluorescence signals in the presence of CB, not of cathepsin l or cathepsin d, due to the CB-specific cleavage of self-quenched peptide probes. Importantly, the intravenously injected CB-CNP demonstrated the potential to discriminate metastases in vivo in three metastatic mouse models, including 4T1-luc2 liver metastases, RFP-B16F10 lung metastases and HT1080 peritoneal metastases. Indeed, Western blot analysis confirmed that the CB expression of metastases had increased compared to nor...Continue Reading

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Citations

May 2, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ju Hee RyuIck Chan Kwon
Feb 11, 2014·Bioconjugate Chemistry·Sangmin LeeKwangmeyung Kim
Dec 29, 2017·Journal of Surgical Oncology·Andrew C PrinceJason M Warram
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Jun 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jingmei YangAo Yu
Sep 21, 2018·Journal of Nanobiotechnology·Jayanta Kumar PatraHan-Seung Shin
Dec 15, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Man Kyu ShimKwangmeyung Kim
Jun 8, 2021·Advanced Functional Materials·Mehdi AziziMichael R Hamblin
Jul 22, 2021·Organic & Biomolecular Chemistry·Kelton A Schleyer, Lina Cui
Feb 15, 2017·Analytical Chemistry·Yuqi WangHong-Yuan Chen
Jan 18, 2018·Analytical Chemistry·Zijuan HaiGaolin Liang

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