Non-invasive quantification of endogenous root auxin transport using an integrated flux microsensor technique

The Plant Journal : for Cell and Molecular Biology
E S McLamoreD M Porterfield

Abstract

Indole-3-acetic acid (IAA) is a primary phytohormone that regulates multiple aspects of plant development. Because polar transport of IAA is an essential determinant of organogenesis and dynamic tropic growth, methods to monitor IAA movement in vivo are in demand. A self-referencing electrochemical microsensor was optimized to non-invasively measure endogenous IAA flux near the surface of Zea mays roots without the addition of exogenous IAA. Enhanced sensor surface modification, decoupling of acquired signals, and integrated flux analyses were combined to provide direct, real time quantification of endogenous IAA movement in B73 maize inbred and brachytic2 (br2) auxin transport mutant roots. BR2 is localized in epidermal and hypodermal tissues at the root apex. br2 roots exhibit reduced shootward IAA transport at the root apex in radiotracer experiments and reduced gravitropic growth. IAA flux data indicates that maximal transport occurs in the distal elongation zone of maize roots, and net transport in/out of br2 roots was decreased compared to B73. Integration of short term real time flux data in this zone revealed oscillatory patterns, with B73 exhibiting shorter oscillatory periods and greater amplitude than br2. IAA efflux...Continue Reading

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Citations

Jan 5, 2012·Analytical and Bioanalytical Chemistry·Fuyou DuHuwei Liu
Mar 17, 2012·Plant & Cell Physiology·Sergio MugnaiStefano Mancuso
Jun 4, 2014·Environmental Science and Pollution Research International·Matthew C StensbergMaria S Sepúlveda
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Jul 20, 2013·The Plant Journal : for Cell and Molecular Biology·Mayandi SivaguruLeon V Kochian
Mar 13, 2014·Physiologia Plantarum·Wendy Ann PeerAngus S Murphy
Jun 20, 2015·The Plant Journal : for Cell and Molecular Biology·Suli YanYingbai Shen
Aug 15, 2012·Biosensors & Bioelectronics·Jin ShiD Marshall Porterfield
Apr 21, 2011·Molecular Plant·Wendy Ann PeerAngus S Murphy
Jan 4, 2012·Plant Signaling & Behavior·Barbara Abraham-Shrauner, Barbara G Pickard
Dec 12, 2012·Plant Signaling & Behavior·František Baluška, Stefano Mancuso
Dec 21, 2010·Analytical Biochemistry·Jin ShiD Marshall Porterfield
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Jul 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yifen ShangJie Zhou
Jun 13, 2017·Annual Review of Analytical Chemistry·Seon-Yeong KwakMichael S Strano
May 4, 2018·Plant Physiology·Maolin ZhangZhaojun Ding

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