Non-invasive Salmonella typhimurium mutants are avirulent because of an inability to enter and destroy M cells of ileal Peyer's patches

Molecular Microbiology
K L PenheiterB D Jones

Abstract

Salmonella typhimurium initiates infection of a host by invading M cells of Peyer's patches within the small intestine. The ability of the bacteria to invade mammalian cells has been shown to be regulated by environmental conditions, including oxygen concentrations, osmolarity, and growth phase. We have previously created oxygen-regulated Tn5lacZY S. typhimurium mutants that are defective in invasion. We have now identified the invasion genes disrupted by eight of the transposon insertions. These genes encode transcriptional regulators (hilA and invF), type III secretory components (orgA, invG and spaR) and secreted proteins (invC and invD). Examination of the protein-secretion profiles of the non-invasive mutants indicated that each of the mutants was defective in secretion of between one and six proteins. We have also demonstrated that the loss of tissue culture cell invasiveness corresponds to an inability to invade and destroy M cells of Peyer's patches in a murine ligated loop model. Virulence studies, performed in mice, demonstrated that these defects significantly reduced the ability of the mutants to cause murine typhoid fever by an oral route of infection. Virulence by an intraperitoneal route of infection was unaffect...Continue Reading

Citations

Nov 26, 2002·Biochemical and Biophysical Research Communications·Peter TyrerAllan Cripps
Jan 16, 2003·Immunology Letters·Mary Jo Wick
Jun 29, 2002·Current Opinion in Immunology·Mary Jo Wick
Feb 27, 1999·Current Opinion in Immunology·J T Harty, M J Bevan
Jul 6, 2000·Trends in Microbiology·R R Isberg, P Barnes
Jul 6, 2000·Trends in Microbiology·A Vazquez-Torres, F C Fang
Oct 21, 1998·Trends in Microbiology·M A Jepson, M A Clark
Jan 5, 2002·Microbes and Infection·M A Jepson, M A Clark
Jan 5, 2002·Microbes and Infection·C P Lostroh, C A Lee
Mar 6, 1999·Current Opinion in Microbiology·P A Cotter, J F Miller
Feb 19, 2000·Current Opinion in Microbiology·A Vazquez-Torres, F C Fang
Feb 27, 1999·Molecular Microbiology·B M AhmerF Heffron
Jun 9, 2000·Molecular Microbiology·T S Wallis, E E Galyov
Jun 9, 2000·Molecular Microbiology·R L Lucas, C A Lee
Aug 20, 2009·The Journal of Biological Chemistry·Joaquín Bernal-Bayard, Francisco Ramos-Morales
Aug 1, 2000·Avian Pathology : Journal of the W.V.P.A·M P Dibb-Fuller, M J Woodward
Jul 19, 2000·The Journal of Experimental Medicine·D M MonackS Falkow
Dec 3, 1999·Immunological Reviews·J C SirardJ P Kraehenbuhl
Jan 5, 2006·Applied and Environmental Microbiology·I GantoisF Van Immerseel

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.