Non-invasive tri-modal visualisation via PET/SPECT/μCT of recombinant human bone morphogenetic protein-2 retention and associated bone regeneration: A proof of concept

Journal of Controlled Release : Official Journal of the Controlled Release Society
Gry Hulsart-BillströmGunnar Antoni

Abstract

Bone morphogenetic proteins (BMP's) are vital for bone and cartilage formation, where bone morphogenetic protein-2 (BMP-2) is acknowledged as a growth factor in osteoblast differentiation. However, uncontrolled delivery may result in adverse clinical effects. In this study we investigated the possibility for longitudinal and non-invasive monitoring of implanted [125I]BMP-2 retention and its relation to ossification at the site of implantation. A unilateral critically sized femoral defect was produced in the left limb of rats while the right femur was retained intact as a paired reference control. The defect was filled with a hyaluronan hydrogel with 25% hydroxyapatite alone (carrier control; n = 2) or combined with a mixture of [125I]BMP-2 (150 μg/ml; n = 4). Bone formation was monitored using micro computed tomography (μCT) scans at 1, 3, 5, 7, 9 and 12 weeks. The retention of [125I]BMP-2 was assessed with single photon emission computed tomography (SPECT), and the bone healing process was followed with sodium fluoride (Na18F) using positron emission tomography (PET) at day 3 and at week 2, 4, and 6. A rapid burst release of [125I]BMP-2 was detected via SPECT. This was followed by a progressive increase in uptake levels of [18...Continue Reading

Citations

Jul 2, 2019·Journal of Tissue Engineering·Eirini A FragogeorgiGeorge Loudos
Mar 26, 2021·Frontiers in Cell and Developmental Biology·Jiqiao YangQing Lv
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Chunqing MengTingfang Sun
Jul 28, 2021·Biological Chemistry·Sabine SchulzeStefan Rammelt

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