Non-invasive, whole-plant imaging of chloroplast movement and chlorophyll fluorescence reveals photosynthetic phenotypes independent of chloroplast photorelocation defects in chloroplast division mutants

The Plant Journal : for Cell and Molecular Biology
Siddhartha DuttaKatherine W Osteryoung

Abstract

Leaf chloroplast movement is thought to optimize light capture and to minimize photodamage. To better understand the impact of chloroplast movement on photosynthesis, we developed a technique based on the imaging of reflectance from leaf surfaces that enables continuous, high-sensitivity, non-invasive measurements of chloroplast movement in multiple intact plants under white actinic light. We validated the method by measuring photorelocation responses in Arabidopsis chloroplast division mutants with drastically enlarged chloroplasts, and in phototropin mutants with impaired photorelocation but normal chloroplast morphology, under different light regimes. Additionally, we expanded our platform to permit simultaneous image-based measurements of chlorophyll fluorescence and chloroplast movement. We show that chloroplast division mutants with enlarged, less-mobile chloroplasts exhibit greater photosystem II photodamage than is observed in the wild type, particularly under fluctuating high levels of light. Comparison between division mutants and the severe photorelocation mutant phot1-5 phot2-1 showed that these effects are not entirely attributable to diminished photorelocation responses, as previously hypothesized, implying that a...Continue Reading

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Citations

Jun 24, 2016·Cell Systems·Jeffrey A CruzDavid M Kramer
Dec 8, 2016·Journal of Theoretical Biology·Kang-Ling LiaoAlan M Jones
Dec 16, 2017·Plant, Cell & Environment·Alejandro MoralesPaul C Struik
Oct 24, 2018·The Plant Journal : for Cell and Molecular Biology·Jiying LiJianping Hu
Jun 25, 2017·Journal of Experimental Botany·Siddhartha DuttaKatherine W Osteryoung
Dec 23, 2017·Frontiers in Plant Science·Yiqiong LiXiaomin Liu
Nov 18, 2016·Royal Society Open Science·Sebastian KuhlgertDavid M Kramer
Jul 20, 2018·Journal of Photochemistry and Photobiology. B, Biology·J SemerM Navrátil
Apr 5, 2021·The Journal of Biological Chemistry·Katie J PorterKatherine W Osteryoung

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