Non-lipolytic and lipolytic sequence-related carboxylesterases: a comparative study of the structure-function relationships of rabbit liver esterase 1 and bovine pancreatic bile-salt-activated lipase

Biochimica Et Biophysica Acta
Henri ChahinianLouis Sarda

Abstract

To differentiate esterases from lipases at the structure-function level, we have compared the kinetic properties and structural features of sequence-related esterase 1 from rabbit liver (rLE) and bile-salt-activated lipase from bovine pancreas (bBAL). In contrast to rLE, bBAL hydrolyses water-insoluble medium and long chain esters as vinyl laurate, trioctanoin and olive oil. Conversely, rLE and bBAL are both active on water-soluble short chain esters as vinyl acetate, vinyl propionate, vinyl butyrate, tripropionin, tributyrin and p-nitrophenyl butyrate. However, the enzymes show distinctive kinetic behaviours. rLE displays maximal activity at low substrate concentration, below the critical micelle concentration, whereas bBAL acts preferencially on emulsified esters, at concentration exceeding the solubility limit. Comparison of the 3D structures of rLE and bBAL shows, in particular, that the peptide loop at positions 116-123 in bBAL is deleted in rLE. This peptide segment interacts with a bile salt molecule thus inducing a conformational transition which gives access to the active site. Inhibition studies and manual docking of a bulky ester molecule as vinyl laurate in the catalytic pocket of rLE and bBAL show that the inabilit...Continue Reading

References

Sep 10, 1992·Nature·H van TilbeurghC Cambillau
Feb 22, 1990·Nature·F K WinklerW Hunziker
Sep 1, 1987·The Biochemical Journal·T Wienker, O von Deimling
May 11, 1973·Molecular and Cellular Biochemistry·W Junge, K Krisch
Nov 26, 1999·Biochimica Et Biophysica Acta·S Longhi, C Cambillau
Dec 2, 2000·Biochimie·M R Egmond, J de Vlieg
Apr 23, 2002·Nature Structural Biology·Sompop BencharitMatthew R Redinbo
Jul 21, 2004·Biochemistry·Yassine Ben AliAbdelkarim Abousalham
Oct 10, 2007·FEBS Letters·Clement AngkawidjajaShigenori Kanaya

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Citations

Apr 26, 2012·International Journal of Biological Macromolecules·Ahmed FendriYoussef Gargouri
May 15, 2012·Biotechnology Advances·Peter Biely
Mar 20, 2014·Toxicology Letters·Georg ReiterFranz Worek
Apr 26, 2016·Chemico-biological Interactions·Nicolas LenfantArnaud Chatonnet

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