Non-muscle myosin IIA differentially regulates intestinal epithelial cell restitution and matrix invasion.

The American Journal of Pathology
Brian A BabbinAndrei I Ivanov

Abstract

Epithelial cell motility is critical for self-rejuvenation of normal intestinal mucosa, wound repair, and cancer metastasis. This process is regulated by the reorganization of the F-actin cytoskeleton, which is driven by a myosin II motor. However, the role of myosin II in regulating epithelial cell migration remains poorly understood. This study addressed the role of non-muscle myosin (NM) IIA in two different modes of epithelial cell migration: two-dimensional (2-D) migration that occurs during wound closure and three-dimensional (3-D) migration through a Matrigel matrix that occurs during cancer metastasis. Pharmacological inhibition or siRNA-mediated knockdown of NM IIA in SK-CO15 human colonic epithelial cells resulted in decreased 2-D migration and increased 3-D invasion. The attenuated 2-D migration was associated with increased cell adhesiveness to collagen and laminin and enhanced expression of beta1-integrin and paxillin. On the 2-D surface, NM IIA-deficient SK-CO15 cells failed to assemble focal adhesions and F-actin stress fibers. In contrast, the enhanced invasion of NM IIA-depleted cells was dependent on Raf-ERK1/2 signaling pathway activation, enhanced calpain activity, and increased calpain-2 expression. Our fin...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Le BivicE Rodriguez-Boulan
Jun 30, 1988·Biochemical and Biophysical Research Communications·T TsujinakaT Mori
Sep 6, 1994·Annals of the New York Academy of Sciences·R E GalardyB Summers
Feb 9, 1996·Cell·D A Lauffenburger, A F Horwitz
Jun 1, 1996·The Journal of Cell Biology·M Chrzanowska-Wodnicka, K Burridge
Apr 1, 1996·Current Opinion in Cell Biology·R Treisman
Mar 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S K Maciver
Feb 1, 1996·Cell Biology International·J P Heath
Jan 1, 1996·Annual Review of Cell and Developmental Biology·K Burridge, M Chrzanowska-Wodnicka
Aug 1, 1997·Clinical Science·A J Wilson, P R Gibson
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·P Friedl, E B Bröcker
Feb 15, 2001·Seminars in Cancer Biology·I Stamenkovic
Mar 16, 2001·Breast Cancer Research : BCR·M J DuffyN O'Higgins
Nov 1, 2001·Neoplasia : an International Journal for Oncology Research·R E Harrison, E A Turley
Nov 27, 2001·Science·E CukiermanK M Yamada
Feb 6, 2002·The Journal of Experimental Medicine·Kevin B HotaryStephen J Weiss
Feb 16, 2002·Biochemical and Biophysical Research Communications·Wen-Xing DingChoon-Nam Ong
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Irina KaverinaJ Victor Small
Sep 17, 2002·Current Opinion in Cell Biology·Edna CukiermanKenneth M Yamada
Oct 16, 2002·The International Journal of Biochemistry & Cell Biology·Neil O Carragher, Margaret C Frame
Feb 21, 2003·International Journal of Cancer. Journal International Du Cancer·Keiran S M Smalley
Mar 15, 2003·Science·Aaron F StraightTimothy J Mitchison
May 14, 2003·Trends in Cell Biology·Frederick Grinnell
Jun 6, 2003·Cancer Metastasis Reviews·Takayuki Shiomi, Yasunori Okada
Jul 9, 2003·The Journal of Biological Chemistry·Mihály KovácsJames R Sellers
Jul 30, 2003·Cancer Metastasis Reviews·Kaladhar B ReddyNatasha Atanaskova
Oct 25, 2003·Journal of Cell Science·Ronen Zaidel-BarBenjamin Geiger
Nov 5, 2003·The Journal of Biological Chemistry·Eliahu GolombRobert S Adelstein
Nov 18, 2003·Molecular Biology of the Cell·Tatsuru Togo, Richard A Steinhardt
Nov 19, 2003·Molecular and Cellular Biochemistry·Sajal ChakrabortiTapati Chakraborti
Dec 6, 2003·Science·Anne J RidleyAlan Rick Horwitz
Jan 7, 2004·Biochemical and Biophysical Research Communications·Mark S DuxburyEdward E Whang
Mar 24, 2004·Current Opinion in Cell Biology·Enrique M De La Cruz, E Michael Ostap
May 26, 2004·Biochemical Society Transactions·R Zaidel-BarB Geiger
Aug 3, 2004·Journal of Cell Science·John W Edmunds, Louis C Mahadevan
Aug 5, 2004·The Journal of Biological Chemistry·Mary Anne ContiRobert S Adelstein
Sep 17, 2004·Journal of Cell Science·Cai HuangMichael D Schaller
Oct 27, 2004·The Biochemical Journal·Matt MacCormickMark L Grimes
Dec 25, 2004·Cellular and Molecular Life Sciences : CMLS·P BastianF Entschladen
Jan 29, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masanori NakayamaKozo Kaibuchi
Mar 19, 2005·The Journal of Biological Chemistry·Jianjun BaoRobert S Adelstein
Mar 26, 2005·Science·Freddy Radtke, Hans Clevers

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Citations

Jun 15, 2011·Virchows Archiv : an International Journal of Pathology·Flavia A WaldPedro J Salas
Nov 12, 2010·American Journal of Physiology. Cell Physiology·Mitulkumar N BavariaLeonard R Johnson
Dec 4, 2012·International Journal of Molecular Sciences·Dongning LiuGuoxin Li
Jun 3, 2011·World Journal of Gastroenterology : WJG·Masahiro Iizuka, Shiho Konno
Oct 1, 2009·Future Medicinal Chemistry·Teresa T BonelloPeter W Gunning
Jun 16, 2010·Cellular and Molecular Bioengineering·Saheli SarkarHarihara Baskaran
Mar 27, 2012·Annals of Diagnostic Pathology·Lucía GonzálezFrancisco J Vizoso
Aug 19, 2011·The FEBS Journal·Shekhar SahaSiddhartha S Jana
Sep 29, 2011·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Z-K XiaY-R Hu
Dec 31, 2009·British Journal of Pharmacology·Zhenan LiuHendrik Reynaert
Jun 29, 2010·The American Journal of Pathology·Andrei I IvanovAsma Nusrat
Dec 28, 2010·Biochemical and Biophysical Research Communications·Shigeru MorimuraKazuhide Takahashi
Oct 10, 2015·Tissue Barriers·Adriana Sartorio GehrenJosé Andrés Morgado-Díaz
Sep 15, 2015·PLoS Neglected Tropical Diseases·C Paul MorrisEdward Mitre
Sep 22, 2016·Cell Cycle·Rashmi PriyaMagdalene Michael
Aug 21, 2018·Cytoskeleton·Aidan M Fenix, Dylan T Burnette
Oct 30, 2018·Journal of Cellular Physiology·Jiaxin ChenXiaotong Hu
Jan 6, 2017·The Journal of Biological Chemistry·Vandana RaiThomas T Egelhoff
Feb 15, 2020·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y GaoS Yuan
Mar 7, 2021·Cancers·Nayden G NaydenovAndrei I Ivanov

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