Non-phosphorylating GAPDH of higher plants is a member of the aldehyde dehydrogenase superfamily with no sequence homology to phosphorylating GAPDH

Journal of Molecular Biology
A HabenichtR Cerff

Abstract

Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDH, NADP-specific, EC 1.2.1.9) operates in the cytosol of autotrophic eukaryotes where it generates NADPH for biosynthetic processes from photosynthetic glyceraldehyde 3-phosphate exported from the chloroplast by the phosphate translocator. Here we report the first cloning and characterization of cDNAs encoding complete polypeptide chains of nonphosphorylating GAPDH from pea and maize by using oligonucleotide probes derived from amino acid sequences determined for the purified enzyme. Unexpectedly, nonphosphorylating GAPDH cannot be aligned with the well-known sequences of phosphorylating GAPDH, but shares about 30% amino acid identity with various specialized and non-specialized aldehyde dehydrogenases (ALDHs) of eubacteria and eukaryotes. A phylogenetic analysis of this ALDH superfamily reveals a complex evolutionary pattern with numerous major branches carrying genes from eubacteria, eukaryotes, or both, encoding enzymes that are specific or non-specific for particular aldehyde substrates. This topology suggests a concomitant emergence of multiple substrate specificities from non-specialized ALDH during an early evolutionary phase of intense metabolic diversifi...Continue Reading

Citations

Feb 22, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·William Martin, Michael J Russell
Apr 21, 1999·Protein Science : a Publication of the Protein Society·J PerozichJ Hempel
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Christopher BräsenBettina Siebers
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Jun 8, 2002·FEMS Microbiology Letters·Abdelghani IddarAbdelaziz Soukri
Mar 20, 2021·Journal of Experimental Botany·Thomas Wieloch
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Jun 23, 2021·The EMBO Journal·Juan C MorenoAleksandra Skirycz
Oct 14, 2008·Chemico-biological Interactions·Sergey A Krupenko

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