Non-POU Domain-Containing Octamer-Binding Protein Negatively Regulates HIV-1 Infection in CD4(+) T Cells

AIDS Research and Human Retroviruses
Corine St GelaisLi Wu

Abstract

HIV-1 interacts with numerous cellular proteins during viral replication. Identifying such host proteins and characterizing their roles in HIV-1 infection can deepen our understanding of the dynamic interplay between host and pathogen. We previously identified non-POU domain-containing octamer-binding protein (NonO or p54nrb) as one of host factors associated with catalytically active preintegration complexes (PIC) of HIV-1 in infected CD4(+) T cells. NonO is involved in nuclear processes including transcriptional regulation and RNA splicing. Although NonO has been identified as an HIV-1 interactant in several recent studies, its role in HIV-1 replication has not been characterized. We investigated the effect of NonO on the HIV-1 life cycle in CD4(+) T cell lines and primary CD4(+) T cells using single-cycle and replication-competent HIV-1 infection assays. We observed that short hairpin RNA (shRNA)-mediated stable NonO knockdown in a CD4(+) Jurkat T cell line and primary CD4(+) T cells did not affect cell viability or proliferation, but enhanced HIV-1 infection. The enhancement of HIV-1 infection in Jurkat T cells correlated with increased viral reverse transcription and gene expression. Knockdown of NonO expression in Jurkat ...Continue Reading

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Citations

Apr 17, 2016·Nucleic Acids Research·Gavin J KnottArcha H Fox
Jul 7, 2018·The Journal of Biological Chemistry·Wuxun LuLi Wu
Nov 13, 2020·Journal of Virology·Constanza E EspadaLi Wu

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Methods Mentioned

BETA
pull-down
flow cytometry
transfection
enzyme-linked immunosorbent assay
ELISA
PCR

Software Mentioned

FlowJo
GraphPad Prism

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