Non-protein coding RNA genes as the novel diagnostic markers for the discrimination of salmonella species using PCR

PloS One
Ravichantar NithyaThean-Hock Tang

Abstract

Salmonellosis, a communicable disease caused by members of the Salmonella species, transmitted to humans through contaminated food or water. It is of paramount importance, to generate accurate detection methods for discriminating the various Salmonella species that cause severe infection in humans, including S. Typhi and S. Paratyphi A. Here, we formulated a strategy of detection and differentiation of salmonellosis by a multiplex polymerase chain reaction assay using S. Typhi non-protein coding RNA (sRNA) genes. With the designed sequences that specifically detect sRNA genes from S. Typhi and S. Paratyphi A, a detection limit of up to 10 pg was achieved. Moreover, in a stool-seeding experiment with S. Typhi and S. Paratyphi A, we have attained a respective detection limit of 15 and 1.5 CFU/mL. The designed strategy using sRNA genes shown here is comparatively sensitive and specific, suitable for clinical diagnosis and disease surveillance, and sRNAs represent an excellent molecular target for infectious disease.

References

Mar 7, 1991·Nature·J Compton
Dec 1, 1994·Journal of Clinical Microbiology·U KongmuangA A Lindberg
Mar 1, 1996·The Journal of Applied Bacteriology·Q ZhuY N Chan
Sep 23, 1997·BioTechniques·O HenegariuP H Vogt
May 23, 2002·Science·Gisela Storz
Mar 20, 2003·Journal of Bacteriology·Wen DengFrederick R Blattner
Sep 27, 2003·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Muhammad Nasrum MassiMasato Kawabata
Mar 9, 2004·Journal of Clinical Microbiology·M BaeleF Haesebrouck
Jul 31, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Apr 27, 2005·Trends in Genetics : TIG·Alexander HüttenhoferNorbert Polacek
Aug 23, 2005·Gene·Fabrício F Costa
Sep 27, 2005·The Lancet Infectious Diseases·Bradley A Connor, Eli Schwartz
Oct 11, 2005·Hematological Oncology·Peter A Hall, Se Hilary Russell
Dec 2, 2005·Emerging Infectious Diseases·R Leon OchiaiJohn D Clemens
Sep 19, 2006·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Bharat M PokharelN R Tuladhar
Dec 14, 2006·International Journal of Environmental Health Research·Anup PalitSujit Kumar Bhattacharya
Feb 13, 2007·Travel Medicine and Infectious Disease·Fiona J Cooke, John Wain
Jun 15, 2007·RNA Biology·Timofey S RozhdestvenskyJürgen Brosius
Oct 30, 2007·Diagnostic Microbiology and Infectious Disease·Ismail AziahAsma Ismail
Nov 21, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Ashish P MaskeyMark D Zimmerman
Feb 10, 2009·Microbiology·Elisabete ValérioRogério Tenreiro
Apr 21, 2009·Applied and Environmental Microbiology·Narjol González-EscalonaKeith A Lampel
Dec 9, 2009·Nucleic Acids Research·Jonathan Livny, Matthew K Waldor
Mar 20, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Aliya NaheedRobert F Breiman
Oct 1, 2010·Molecular Cancer Research : MCR·Subash C B GopinathPenmetcha K R Kumar
Oct 15, 2010·Journal of Cellular Physiology·Yexin Zhou, Jianping Xie
May 10, 2011·Trends in Cell Biology·Orly Wapinski, Howard Y Chang
May 26, 2011·RNA·Carsten A RaabeTimofey S Rozhdestvensky
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Jul 12, 2012·Thrombosis and Haemostasis·Seema Dangwal, Thomas Thum
Sep 13, 2012·Sensors·Chia-Chen ChangChii-Wann Lin
May 1, 2010·Expert Opinion on Medical Diagnostics·Joel A Lefferts, Gregory J Tsongalis

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Datasets Mentioned

BETA
FJ746361.1

Methods Mentioned

BETA
PCR
electrophoresis
nucleic acid amplification

Software Mentioned

mfold

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