Non-proton-motive-force-dependent sodium efflux from the ruminal bacterium Streptococcus bovis: bound versus free pools.

Applied and Environmental Microbiology
H J Strobel, J B Russell

Abstract

Growing cells of Streptococcus bovis JB1 had a sodium content of 1,125 nmol/mg of protein and, based on a ratio of cell volume to protein of 4.3 microliters/mg, the apparent intracellular sodium concentration was more than 240 mM. Much of this sodium could not be removed by water washing even if cells were boiled or treated with the pore-forming ionophore, gramicidin, but it could be exchanged for potassium. Stationary cultures had a 2.6-microliters volume per milligram of protein and a total sodium content of 410 mM. When stationary cultures were energized with glucose at pH 6 to 8, sodium (more than 200 mM) was expelled within 2 min, and it appeared that growing cells had a very small pool of free intracellular sodium. Sodium-proton antiport activity could not be demonstrated with a sodium pulse, and the protonophore SF6847, valinomycin, and the H+-ATPase inhibitor dicyclohexylcarbodiimide (DCCD) had little effect on sodium efflux, even though these inhibitors greatly reduced the proton-motive force. SF6847, valinomycin, and DCCD had little effect on intracellular ATP, but iodoacetate, an inhibitor of glycolysis, decreased ATP as well as sodium efflux. Stationary cells from sodium-deficient medium expelled little sodium after...Continue Reading

References

Sep 1, 1987·Journal of Bacteriology·Y Kakinuma
Oct 1, 1989·Applied and Environmental Microbiology·G J Chen, J B Russell
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·P C MaloneyT H Wilson
Mar 1, 1974·Applied Microbiology·D R Caldwell, R F Hudson
Jul 1, 1984·Journal of Dairy Science·J B Russell, P H Robinson

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Citations

Oct 1, 1990·Journal of Dairy Science·J B RussellS A Martin
Feb 11, 2004·FEMS Microbiology Letters·Adam J HoulihanJames B Russell
Jan 1, 1990·Archives of Microbiology·J B Russell, H J Strobel
Jan 4, 2003·Journal of Bacteriology·Karen OlssonGregory M Cook
May 29, 1999·Applied and Environmental Microbiology·H Kajikawa, S Masaki
Oct 1, 1989·Applied and Environmental Microbiology·G J Chen, J B Russell
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·Y Kakinuma
Jun 1, 1990·Applied and Environmental Microbiology·J M Chow, J B Russell
Dec 1, 1996·Applied and Environmental Microbiology·R P Lana, J B Russell
May 1, 1997·Applied and Environmental Microbiology·H KajikawaS Masaki

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