PMID: 8947529Nov 1, 1996Paper

Non-random distribution of DNA double-strand breaks induced by particle irradiation

International Journal of Radiation Biology
M LöbrichB Rydberg

Abstract

Induction of DNA double-strand breaks (dsbs) in mammalian cells is dependent on the spatial distribution of energy deposition from the ionizing radiation. For high LET particle radiations the primary ionization sites occur in a correlated manner along the track of the particles, while for X-rays these sites are much more randomly distributed throughout the volume of the cell. It can therefore be expected that the distribution of dsbs linearly along the DNA molecule also varies with the type of radiation and the ionization density. Using pulsed-field gel and conventional gel techniques, we measured the size distribution of DNA molecules from irradiated human fibroblasts in the total range of 0.1 kbp-10 Mbp for X-rays and high LET particles (N ions, 97 keV/microns and Fe ions, 150 keV/microns). On a mega base pair scale we applied conventional pulsed-field gel electrophoresis techniques such as measurement of the fraction of DNA released from the well (FAR) and measurement of breakage within a specific NotI restriction fragment (hybridization assay). The induction rate for widely spaced breaks was found to decrease with LET. However, when the entire distribution of radiation-induced fragments was analysed, we detected an excess o...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H IchikawaC R Cantor
Nov 1, 1988·International Journal of Radiation Biology·D Blöcher
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·R K SachsJ E Hearst
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Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·M LöbrichP K Cooper

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Citations

Feb 12, 2002·Radiation Research·Jürgen KieferSamuel Ikpeme
Oct 14, 2003·International Journal of Radiation Biology·A HolgerssonA E Meijer
Sep 29, 2005·Radiation Research·Valentina DiniMaria Antonella Tabocchini
Nov 7, 2008·Journal of Radiation Research·Akihisa TakahashiTakeo Ohnishi
Oct 4, 2012·Health Physics·Maria Antonella TabocchiniValentina Dini
Aug 7, 2012·Journal of Mathematical Biology·Yasumasa Saisho, Atsushi Ito
May 21, 2013·Radiation Research·Alexandros G GeorgakilasRobert D Stewart
Jun 5, 2015·Journal of Cellular Physiology·Wendy J Cannan, David S Pederson
May 7, 2016·Acta Biochimica Et Biophysica Sinica·Yi-Li FengAn-Yong Xie
Nov 12, 2017·Radiation and Environmental Biophysics·Qianlin CaoHongying Yang
Mar 9, 2002·Molecular and Cellular Biology·Tsutomu ShimuraOhtsura Niwa
Jun 10, 2003·Journal of Radiation Research·Mauro BelliMaria Antonella Tabocchini
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J F Ward
Nov 18, 2006·Physics in Medicine and Biology·C-K Chris Wang, Xin Zhang
Aug 10, 2011·Radiation Research·Robert D StewartDavid J Carlson
Jul 26, 2011·Journal of Radiation Research·Teruaki KonishiKotaro Hieda
Dec 22, 2010·International Journal of Radiation Biology·Kristina ClaessonKecke Elmroth
Apr 17, 2008·Journal of Radiation Research·Megumi Hada, Alexandros G Georgakilas
Oct 31, 2012·Scientific Reports·Xin ZhouHong Zhang
Sep 8, 2018·Oncotarget·Lara CoblerLuika A Timmerman
Mar 11, 2020·Frontiers in Molecular Biosciences·Alexandra C VítorSérgio F de Almeida

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