Non random distribution of lesions induced by deoxyribonuclease I in human chromosomes.

Cytotechnology
F NuzzoE Raimondi

Abstract

We studied the action of deoxyribonuclease I on human lymphocytes in order to determine the localization of the DNAase induced aberrations. Our results indicate a non-random distribution of the lesions on chromosome regions which may reflect a differential pattern of sensitivity to the enzyme. Furthermore we observed a correspondence between the preferential DNAase induced breaks and fragile sites that are expressed in lymphocytes maintained in medium without folic acid. A possible interpretation of our findings is that the accessibility to DNAase and/or the efficiency of the repair systems depend on the chromatin structure that influences also the expression of some common fragile sites.

References

Dec 6, 1978·Chromosoma·C G SahasrabuddheT C Hsu
Sep 3, 1976·Science·H Weintraub, M Groudine
Jun 1, 1985·Mutation Research·G ObeR De Salvia
Jun 1, 1986·Experimental Cell Research·G D Burkholder, G J Schmidt
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M T Kuo, W Plunkett
Jan 1, 1985·Cytogenetics and Cell Genetics·R BergerG R Sutherland
Jan 1, 1985·Human Genetics·M De BraekeleerC C Lin
Jan 1, 1973·Cytogenetics and Cell Genetics·H Von Koskull, P Aula
Aug 1, 1965·Journal of Molecular Biology·A D BanghamJ C Watkins
Jul 1, 1984·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·P E Bryant
Dec 1, 1981·Cell·S C Elgin
Dec 7, 1984·Science·J J Yunis, A L Soreng
Jan 1, 1983·Cytogenetics and Cell Genetics·R MezzanotteL Ferrucci
Feb 1, 1981·Experimental Cell Research·R Gagné

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.