Jul 21, 2014

Non-respiratory functions of Saccharomyces cerevisiae mitochondria are required for optimal attractiveness to Drosophila melanogaster

BioRxiv : the Preprint Server for Biology
Kelly M SchiaborMichael Eisen

Abstract

While screening a large collection of wild and laboratory yeast isolates for their ability to attract Drosophila melanogaster adults, we noticed a large difference in fly preference for two nearly isogenic strains of Saccharomyces cerevisiae , BY4741 and BY4742. Using standard genetic analyses, we tracked the preference difference to the lack of functional mitochondria the stock of BY4742 used in the initial experiment. We used gas chromatography coupled with mass spectroscopy to examine the volatile compounds produced by BY4741 and the mitochondria-deficient BY4742, and found they differed significantly. We found that several ethyl esters are present at much higher levels in strains with functional mitochondria, even in fermentative conditions. We confirmed the role of these ethyl esters in attraction by examining an EEB1Δ strain which reduces ethyl ester production. We found that nitrogen levels in the substrate affect the production of these compounds, and that they are produced at high levels by strains with functional mitochondria in the fermentation of natural substrates. Collectively these observations demonstrate the effect core metabolic processes have in mediating the interaction between yeasts and insect vectors, and...Continue Reading

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Mentioned in this Paper

Saccharomyces cerevisiae allergenic extract
Yeasts
Drosophila
Insect Vectors
Laboratory
Wild bird
Mass Spectrometry
Volatile Substances
Mitochondria
Nitrogen

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