PMID: 3604518Feb 1, 1987

Non-respiring rat liver mitochondria do not have a Ca2+/2H+ antiporter

Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry
N-E L Saris


Liver mitochondria take up Ca2+ by the Ca2+ uniporter, whereas at steady state efflux is believed to occur mainly by means of a ruthenium red-insensitive Ca2+/2H+ antiporter. The latter activity was studied in respiration-inhibited mitochondria in the presence of ruthenium red and was measured as Ca2+ uptake following acidification of the matrix by addition of nigericin, which catalyzes K+/H+ exchange. Ca2+ uptake was stimulated by protonophorous uncoupling agents and inhibited by increasing the concentration of ruthenium red. However, the rates were always smaller than those obtained by addition of valinomycin instead of nigericin. This indicates that under these conditions, Ca2+ fluxes are not mediated by a Ca2+/2H+ antiporter but by residual uniporter activity.


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Jan 18, 2002·Journal of Neurochemistry·C S CasleyM A Sharpe
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Dec 9, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·L CanevariB K Siesjö
Jul 6, 2000·British Journal of Pharmacology·J Zheng, V D Ramirez
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