Non-retroviral Endogenous Viral Element Limits Cognate Virus Replication in Aedes aegypti Ovaries.

Current Biology : CB
Yasutsugu SuzukiMaria-Carla Saleh

Abstract

Endogenous viral elements (EVEs) are viral sequences integrated in host genomes. A large number of non-retroviral EVEs was recently detected in Aedes mosquito genomes, leading to the hypothesis that mosquito EVEs may control exogenous infections by closely related viruses. Here, we experimentally investigated the role of an EVE naturally found in Aedes aegypti populations and derived from the widespread insect-specific virus, cell-fusing agent virus (CFAV). Using CRISPR-Cas9 genome editing, we created an Ae. aegypti line lacking the CFAV EVE. Absence of the EVE resulted in increased CFAV replication in ovaries, possibly modulating vertical transmission of the virus. Viral replication was controlled by targeting of viral RNA by EVE-derived P-element-induced wimpy testis-interacting RNAs (piRNAs). Our results provide evidence that antiviral piRNAs are produced in the presence of a naturally occurring EVE and its cognate virus, demonstrating a functional link between non-retroviral EVEs and antiviral immunity in a natural insect-virus interaction.

Citations

Sep 10, 2020·Viruses·Tongtong Zhu, Ana Fernandez-Sesma
Jul 30, 2020·Nature Reviews. Microbiology·Ursula Hofer
Feb 2, 2021·Frontiers in Cellular and Infection Microbiology·Vanessa M MaciasJason L Rasgon
Jan 13, 2021·Nature Reviews. Microbiology·Scott C WeaverNikos Vasilakis
Jan 22, 2021·Developmental and Comparative Immunology·Samara Rosendo MachadoPascal Miesen
Feb 25, 2021·Transcription·Nayef Jarrous, Alexander Rouvinski
May 4, 2021·Journal of Medical Entomology·Melisa B BonicaMaría V Micieli
May 16, 2021·Current Opinion in Virology·João Pp de AlmeidaJoão T Marques
Jun 21, 2021·Trends in Parasitology·Rebecca HalbachRonald P van Rij
Aug 19, 2021·PLoS Pathogens·Tarcísio Fontenele de BritoAttilio Pane
Nov 3, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Matheus A DuarteBergmann M Ribeiro
Nov 7, 2021·Current Opinion in Insect Science·Umberto PalatiniMariangela Bonizzoni

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRR01437595
PRJNA588447
SRR5562867

Methods Mentioned

BETA
PCR
Infection
Knockout
genotyping
in vitro transcription
RNA-seq
DNA Assay
transfection
electrophoresis

Software Mentioned

Bowtie2
bedtools
Graphpad Prism
FastQC
Bioconductor
SAMtools
R
Geneious
SRA Toolkit
BLAST

Related Concepts