Non-selective regulation of peroxide and superoxide resistance genes by PerR in Campylobacter jejuni

Frontiers in Microbiology
Jong-Chul KimByeonghwa Jeon

Abstract

Campylobacter jejuni is an important foodborne pathogen. The molecular mechanisms for the regulation of oxidative stress resistance have not yet been understood fully in this bacterium. In this study, we investigated how PerR (peroxide stress regulator) modulates the transcriptional regulation of both peroxide and superoxide resistance genes in C. jejuni, particularly under oxidative stress conditions. The transcriptional levels of ahpC, katA, and sodB were substantially increased by aeration and oxidant exposure. Interestingly, a perR mutation completely abrogated the transcriptional response of ahpC, katA and sodB to oxidants. Furthermore, we demonstrated that perR transcription was reduced by aeration and oxidant exposure. In contrast to the unique role of PerR homologs in peroxide stress regulation in other bacteria, C. jejuni PerR directly regulates the transcription of sodB, the most important gene in superoxide defense, as evidenced by the alteration of sodB transcription by the perR mutation and direct binding of rPerR to the sodB promoter. In addition, we also observed notable morphological changes in C. jejuni from spiral rods to cocoid morphology under aerobic conditions. Based on the intracellular ATP levels, C. jej...Continue Reading

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Citations

Feb 6, 2021·World Journal of Microbiology & Biotechnology·Laurens MaertensRob Van Houdt
Feb 23, 2021·Frontiers in Cellular and Infection Microbiology·Abdi ElmiOzan Gundogdu
May 18, 2021·Frontiers in Immunology·Ananya Sen, James A Imlay

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Methods Mentioned

BETA
Assay
PCR
Cleavage Capture

Software Mentioned

GraphPad
GraphPad Prism

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Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.