Non-specific phospholipase C4 mediates response to aluminum toxicity in Arabidopsis thaliana

Frontiers in Plant Science
Přemysl PejcharJan Martinec

Abstract

Aluminum ions (Al) have been recognized as a major toxic factor for crop production in acidic soils. The first indication of the Al toxicity in plants is the cessation of root growth, but the mechanism of root growth inhibition is largely unknown. Here we examined the impact of Al on the expression, activity, and function of the non-specific phospholipase C4 (NPC4), a plasma membrane-bound isoform of NPC, a member of the plant phospholipase family, in Arabidopsis thaliana. We observed a lower expression of NPC4 using β-glucuronidase assay and a decreased formation of labeled diacylglycerol, product of NPC activity, using fluorescently labeled phosphatidylcholine as a phospholipase substrate in Arabidopsis WT seedlings treated with AlCl3 for 2 h. The effect on in situ NPC activity persisted for longer Al treatment periods (8, 14 h). Interestingly, in seedlings overexpressing NPC4, the Al-mediated NPC-inhibiting effect was alleviated at 14 h. However, in vitro activity and localization of NPC4 were not affected by Al, thus excluding direct inhibition by Al ions or possible translocation of NPC4 as the mechanisms involved in NPC-inhibiting effect. Furthermore, the growth of tobacco pollen tubes rapidly arrested by Al was partially...Continue Reading

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Citations

Jan 20, 2016·Progress in Lipid Research·Yueyun HongXuemin Wang
Mar 19, 2016·The Plant Journal : for Cell and Molecular Biology·Huasheng CaoXuemin Wang
Apr 27, 2019·Frontiers in Plant Science·Tomáš TakáčJozef Šamaj
Jun 3, 2021·Plants·Víctor M González-MendozaS M Teresa Hernández-Sotomayor
May 23, 2021·Plant & Cell Physiology·Di YangYan Wu

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

JMicroVision

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